汇集白膜层制备单人份血浆悬浮去白混合浓缩血小板应用研究  

The applied research of pooled leukocyte-poor platelet concentrate prepared by pooling with buffy coats

作　　者：赵凤绵[1] 戚海[1] 张爱红[1] 牛宏伟[1] 韩卫[1] 刘敬闪[1] 李建民[1] 陈筱麟[1] 张金彩[1] 陈红霞[1] 

机构地区：[1]河北省血液中心,石家庄市050071

出　　处：《河北医药》2015年第5期645-648,共4页Hebei Medical Journal

基　　金：国家卫生公益性行业科研专项(编号:200902008-03);河北省科学技术研究与发展重点课题(编号:20110270)

摘　　要：目的考察用汇集白膜层方法制备去白混合浓缩血小板的质量、功能和代谢产物的变化。方法从400 ml/袋新鲜全血中分离出白膜层(BC),将7袋同血型的BCs混合在一起,加入其中一名男性献血者的血浆200 ml,离心、分离出混合浓缩血小板,用两种国产血小板过滤器过滤(试验Ⅰ组和试验Ⅱ组),测定去白混合浓缩血小板的血小板计数、红细胞和白细胞残留量,血小板聚集功能、活化标志物CD62p、抗低渗休克反应(HSR)、p H值、葡萄糖(mmol/L)、乳酸盐、PCO2(mm Hg)、PO2(mm Hg)等,用单采血小板作对照。结果试验I组和试验Ⅱ组:血小板回收率>90%,血小板含量≥2.5×1011/袋,白细胞混入量<0.15×106/袋;HSR分别为(72.34±40.05)和(43.75±23.53);CD62p分别为(6.10±1.60)和(5.56±3.50);ADP诱导的聚集率(单位Ω)分别为(11.25±8.50)和(8.30±3.83),胶原诱导的聚集率(单位Ω)分别为(18.33±6.42)和(18.87±6.18);p H值、葡萄糖(mmol/L)、乳酸盐、PCO2(mm Hg)、PO2(mm Hg)分别为(6.71±0.13)和(6.49±0.17)、(19.84±3.76)和(19.79±1.36)、(8.98±3.28)和(6.83±3.20)、(71.05±33.32)和(113.70±48.99)、(39.61±42.13)和(57.43±45.43)。结论该工艺制备的产品质量符合国家有关标准,体外功能和贮存代谢变化在可接受的范围内。Objective To evaluate the quality,vitro function and metabolite changes of pooled leukocyte-poor plateletconcentrate prepared by pooling with buffy coats. Methods The buffy coat （BC） was separated by pooling from 400ml freshwhole blood. Seven bag of BCs with same blood type were pooled into a 200ml plasma, which was from a male donor. Themixtures were centrifugated and separated to get pooled platelet concentrate, which were fihered separately through two kinds ofdomestic platelet filters （ group I and group 11 ） from white blood cells （WBC）. The final products was pooled leukocyte-poorplatelet concentrates. The platelet count, erythrocyte and WBC residues, platelet aggregation function, activation marker CD62p,resistance to hypotonic shock response （ HSR）, pH, glucose （ mmol/L）, lactate, PCO2 （ mm Hg）, PO2 （ mm Hg） weredetected, with single collection platelet as controls. Results The group I and group 11 platelet recovery rate 〉 90% ,plateletcontent 〉2.5 x 10n/bag,WBC mixing volume 〈 0. 15 x 106/bag; HSR was 72.34± 40.05 and 43.75 ±23.53 ,respectively;CD62p was 6.10 ± 1.60 and 5.56 ±3.50,respectively;ADP induced platelet aggregation rate （units of Ω） was 11.25± 8.50and 8.30 ±3.83,respectively;collagen induced platelet aggregation rate （units of Ω） was 18.33 ±6.42 and 18.87 ±6.18,respectively ; pH, glucose, lactate （ mmol/L）, PCO2 （ mm Hg）, PO2 （mm Hg） in group I and Ⅱ were 6.71 ± 0.13 vs 6.49 ±0.17,19.84±3.76 vs 19.79± 1.36,8.98 ±3.28 vs 6.83±3.20,71.05 ±33.32 vs 113.70 ±48.99,39.61 ±42. 13 vs57.43± 45.43,respectively. Conclusion The production quality prepared by the technique is accordant with national relatedcriteria, and the changes of vitro function and metabolism are in the acceptable range.

关 键 词：去白混合浓缩血小板 汇集白膜层 血浆悬浮 电阻法血小板聚集试验 

分 类 号：R893.1[医药卫生—法医学]