与大豆根部健康和胞囊线虫侵染相关的根际细菌群落  

Rhizosphere bacterial communities associated with healthy and Heterodera glycines-infected soybean roots

作　　者：宋洁 Yingbo Zhu Jianqing Tian Fengyu Shi Lei Su Keke Liu Meichun Xiang Xingzhong Liu 

机构地区：[1]State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China [2]College of Life Science, Hebei Normal University of Science and Technology, Hebei, Qinhuangdao 066600, PR China [3]Department of Plant Pathology, China Agricultural University, Beijing 100193, PR China

出　　处：《大豆科技》2015年第1期35-35,共1页Soybean Science & Technology

摘　　要：采用Biolog和16S r DNA克隆文库分析方法研究了3种处理的大豆植株的根际土壤细菌群落,包括健康大豆植株（healthy）、被大豆胞囊线虫（Heterodera glycines）侵染的大豆植株（SCN）和被大豆胞囊线虫侵染的,但用淡紫拟青霉（Purpureocillium lilacinus）YES-2菌株处理（SCN＋淡紫拟青霉）的大豆植株。Biolog数据分析结果显示,3个处理之间根际细菌群落的底物利用方式差异显著，并且健康大豆植株的底物丰富度和代谢多样性最低。Bacterial communities in rhizosphere soil of soybean plants that were healthy, infected with soybean cyst nema- tode （SCN, Heterodera glycines）, and infected with SCN but treated with Purpureocillium lilacinus YES-2 were investi- gated with community-level physiological profile （Biolog） and 16S rDNA clone library analyses. Biolog data indicated significant differences in substrate utilization patterns of the rhizosphere bacterial communities associated with healthy, SCN-infected, and SCN-infected plus P. lilacinus-treated plants; among the three treatments, substrate richness and catabolic diversity were lowest in the rhizosphere of healthy soybeans. Analysis of 16S rDNA profiles placed the soy- bean rhizosphere bacteria into seven groups： Proteobacteria, Actinobacteria, Acidobacteria, Firmicutes, Bacteroidetes, Chloroflexi, and an unclassified bacterial clade. The percentages of Rhizobiales and Actinobacteria clones were greater in the rhizosphere of healthy plants than SCN-infected plants, while the opposite was true for the proportions of Bacte- roidetes and Firmicutes clones. Addition of P. lilacinus did significantly affect the rhizosphere bacterial community of SCN-infected plants. These results suggest that rhizosphere bacterial community may play an important role in the changes of soybean rhizosphere biological conditions during the infection process. Further studies will identify more specific changes in the rhizosphere bacterial community during the establishment and progression of SCN disease, and relate these changes to potential effects on disease management, soybean health, and soybean productivity.

关 键 词：土壤细菌群落 大豆胞囊线虫 大豆植株 根际 侵染 健康 Biolog 淡紫拟青霉 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]