早期常压高氧治疗对大鼠颅脑损伤后线粒体功能及细胞凋亡的影响  

Effect of early normobaric hyperoxia therapy on mitochondrial function and apoptosis after craniocerebral trauma in rats

作　　者：朱岁军[1] 刘晨[2] 王俊[1] 唐超[1] 单华[1] 李伟[1] Rajneesh Mungur 沈建[2] 

机构地区：[1]杭州师范大学附属余杭医院神经外科,杭州311100 [2]浙江大学医学院附属第一医院脑医学中心实验室,杭州310006 [3]浙江大学医学院,杭州310009

出　　处：《中华危重症医学杂志（电子版）》2015年第1期17-22,共6页Chinese Journal of Critical Care Medicine:Electronic Edition

基　　金：杭州市医药卫生科技计划项目(20110833B50)

摘　　要：目的探讨早期常压高氧治疗对大鼠颅脑损伤后线粒体功能及细胞凋亡的保护作用及其机制。方法将成年健康雄性大鼠分为假手术组、模型组、高压氧组及常压氧组,每组28只。于伤后1周行神经功能缺损评分,并检测线粒体膜电位(MMP)、丙二醛(MDA)和超氧化物歧化酶(SOD)的变化。观察各组电镜下线粒体形态,并采用原位缺口末端标记(TUNEL)法检测神经细胞凋亡情况。结果四组间神经功能缺损评分比较差异有统计学意义(F=3.682,P=0.039),且常压氧组与高压氧组较模型均明显改善(P均<0.05)。四组间MMP、MDA、SOD及阳性细胞计数比较,差异均有统计学意义(F=8.571、12.4、6.604、9.425,P均<0.05),与模型组比较,常压氧组及高压氧组的MMP均明显升高,MDA、SOD及阳性细胞计数均明显下降(P均<0.05),而高压氧组与常压氧组的MMP、MDA、SOD及阳性细胞计数比较,差异均无统计学意义(P均>0.05)。电镜下,模型组线粒体膜出现断裂、形态较大、肿胀明显,线粒体内出现空泡化,线粒体嵴消失;而常压氧及高压氧组线粒体膜基本完整,肿胀程度较模型组轻微,线粒体嵴尚存在,未见明显空泡化现象,且后两者线粒体形态差异不明显。结论早期常压氧治疗通过保护线粒体结构和功能从而抑制缺血半影区的细胞凋亡,以促进大鼠脑外伤后的神经功能的恢复。Objective To investigate the protective effect and its mechanism of early normobaric hyperoxia therapy on mitochondrial function and apoptosis after craniocerebral trauma in rats. Methods Adult male SD rats were randomly divided into the sham operation group,model group, normobaric hyperoxia group and hyperbaric oxygen group, 28 rats in each group.The neurological severity score, mitochondrial membrane potential（MMP）, malondialdehyde（MDA）and superoxide dismutase（SOD） were detected, and the mitochondrial morphology was observed by electron microscopy in ischemic penumbra seven days after injury. The Td T-mediated d UTP nick end labeling（TUNEL） was used to test the neural cell apoptosis.Results There were significant differences of the neurological severity score among the four groups（F = 3.682, P = 0.039）, and the scores in the normobaric hyperoxia and hyperbaric oxygen groups were better than that in the model group（all P 0.05）. The levels of MMP, MDA and SOD and TUNEL positive cells were significantly different among the four groups（F = 8.571,12.400, 6.604, 9.425; all P 0.05）. In the normobaric hyperoxia and hyperbaric oxygen groups,the levels of MMP were lower, the levels of MDA and SOD and TUNEL positive cells were higher than those in the model group（all P 0.05）. However, the levels of MMP, MDA and SOD and TUNEL positive cells had no statistical significance between the normobaric hyperoxia and hyperbaric oxygen groups（all P 0.05）. In the mitochondrial morphology, mitochondrial membrane were ruptured, larger, swelling, cavitation occurred, and mitochondrial cristae disappeared in the model group. In the normobaric hyperoxia and hyperbaric oxygen groups,mitochondrial membrane were complete and mild swelling, the mitochondrial crest still existed and no obvious cavitation. Conclusion Early normobaric hyperoxia therapy can promote the recovery of neurological function after craniocerebral trauma in rats, and its possible mechanism is to inhibit apoptosis in ischemic penumbra b

关 键 词：常压高氧 颅脑损伤 线粒体 细胞凋亡 

分 类 号：R651.15[医药卫生—外科学]