野生白木香叶乙酸乙酯提取物化学成分的分离鉴定  

Isolation and Identification of the Chemical Constituents in Ethyl Acetate Extracts of Wild Aquilaria sinensis Leaves

作　　者：杨懋勋[1,2] 梁耀光 陈河如[1,4] 黄永芳[5] 龚海光[5] 

机构地区：[1]暨南大学药学院中药及天然药物研究所,广东广州510632 [2]中山火炬职业技术学院,广东中山528436 [3]中山优诺生物科技发展有限公司,广东中山528400 [4]广东省中药药效物质基础及创新药物研究重点实验室,广东广州510632 [5]华南农业大学林学院,广东广州510642

出　　处：《现代食品科技》2015年第2期128-132,163,共6页Modern Food Science and Technology

基　　金：国家自然科学基金(81172982);广东省科技计划项目(2010A030100006);中山市科技计划项目(20101H006;20123A276)

摘　　要：为探究沉香茶系列保健食品的主要原料白木香(Aquilaria sinensis)叶片药理活性的物质基础,本研究通过含70%丙酮的水溶液渗漉提取、石油醚和乙酸乙酯多次萃取后,运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱和制备高效液相色谱等技术从野生白木香叶片乙酸乙酯萃取部位中分离纯化得到7个化合物,根据MS、NMR等波谱数据分别鉴定其中6个化合物为麦角甾醇(1)、槲皮素-3-O-β-D-吡喃半乳糖苷(2)、豆甾醇(3)、5,4'-二羟基-7-甲氧基二氢黄酮(4)、谷甾醇(5)、槲皮素-3-O-β-D-吡喃葡萄糖苷(6)。6个化合物按照结构可分为甾醇及其衍生物类(3个),黄酮苷类(2个)和二氢黄酮类(1个)。其中化合物1-2为首次在瑞香科植物中分离得到,化合物4是在瑞香科沉香属植物中首次分离得到的二氢黄酮类化合物,并首次报道了该化合物的2D NMR数据及归属。The aim of this study was to explore the material basis for the pharmacological activities of Aquilaria sinensis leaves, which are the main components of the ‘Aquilaria Tea＇ health food product series. Dried leaf powder was percolated with an aqueous solution containing 70% acetone and extracted several times using petroleum ether and ethyl acetate. Subsequently, seven compounds were isolated from the ethyl acetate extracts of wild Aquilaria sinensis leaves by multiple techniques including silica gel column chromatography, gel filtration chromatography（Sephadex LH-20）, and preparative high performance liquid chromatography（PHPLC）. Data from mass spectrometry（MS） and nuclear magnetic resonance（NMR） spectroscopy revealed the presence of six compounds： ergosterol（1）, quercetin-3-O-β-Dgalactopyranoside（2）, stigmasterol（3）, 5,4-dihydroxy-7-methoxyflavanone（4）, β-sitosterol（5）, and quercetin-3-O-β-D-glucopyranoside（6）. Based on their structures, the compounds could be classified as three sterols and their derivatives, two flavonoid glycosides, and one flavanone. Among them, compounds 1-2 were first isolated from the family Thymelaeaceae, and compound 4 was the first flavanone isolated from the genus Aquilaria（family Thymelaeaceae）. The 2D NMR data and chemical shift assignments of compound 5 were reported here for the first time.

关 键 词：野生白木香 化学成分 甾醇 黄酮苷 二氢黄酮 

分 类 号：R284[医药卫生—中药学]