当归芍药散对肝硬化腹水大鼠血管紧张素Ⅱ、内皮素-1的影响  

Effects of Danggui Shaoyao Powder on AngiotensinⅡand Endothelin-1 Levels in Rats with Cirrhotic Ascites

作　　者：岳晓莉[1] 王成业[1] 姚瑶[1] 蔡衡[1] 潘永福[1] 王运来[1] 许钒[1] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230012

出　　处：《安徽中医药大学学报》2015年第1期59-61,共3页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81173368)

摘　　要：目的观察当归芍药散对肝硬化腹水大鼠血浆血管紧张素Ⅱ(angiotensin-Ⅱ,AngⅡ)和内皮素-1(endothelin-1,ET-1)水平的影响。方法将雄性SD大鼠随机分为正常组,模型组,甘利欣组,当归芍药散高、中、低剂量组。采用苯巴比妥灌胃联合四氯化碳腹腔注射复制肝硬化腹水模型。采用酶联免疫吸附试验检测血浆AngⅡ、ET-1水平。结果与正常组比较,模型组大鼠凝血酶原时间显著延长(P<0.05),血浆AngⅡ和ET-1水平显著升高(P<0.05)。与模型组比较,甘利欣组和当归芍药散各剂量组凝血酶原时间均显著缩短(P<0.05),血浆AngⅡ和ET-1水平显著降低(P<0.05)。当归芍药散各剂量组及甘利欣组之间凝血酶原时间及血浆AngⅡ、ET-1水平相比较,差异均无统计学意义(P>0.05)。结论当归芍药散治疗肝硬化腹水的机制与降低血浆AngⅡ、ET-1水平有关。Objective To investigate the effects of Danggui Shaoyao Powder（DSP）on the plasma levels of angiotensin Ⅱ（Ang Ⅱ）and endothelin-1（ET-1）in rats with cirrhotic ascites.Methods Male Sprague-Dawley rats were randomly assigned to normal group,model group,Ganlixin group,and DSP groups which were further divided into high-dose,middle-dose,and low-dose groups.Intragastric administration of phenobarbital and intraperitoneal injection of carbon tetrachloride were adopted to establish a rat model of cirrhotic ascites.Prothrombin time（PT）was measured by a blood coagulation analyzer.The plasma levels of Ang Ⅱ and ET-1 were measured by enzyme-linked immunosorbent assay.Results Compared with the normal group,the model group had significant increases in PT and AngⅡ and ET-1levels（P〈0.05）.Compared with the model group,the Ganlixin and DSP groups had significant decreases in PT and Ang Ⅱ and ET-1levels（P〈0.05）.PT and AngⅡ and ET-1levels showed no significant differences between the DSP and Ganlixin groups（P〉0.05）.Conclusion DSP could reduce plasma levels of Ang Ⅱand ET-1,which may be related to its action mechanism in the treatment of cirrhotic ascites.

关 键 词：当归芍药散 肝硬化腹水 血管紧张素Ⅱ 内皮素-1 

分 类 号：R657.31[医药卫生—外科学] R285.5[医药卫生—临床医学]