欧洲型猪繁殖与呼吸综合征病毒辽宁分离株全基因测序与遗传演化分析  

Genomic sequencing and phylogenetic analysis of EU-PRRSV isolated in Liaoning Province

作　　者：李冰[1,2] 高慎阳[1] 卢赫[1] 冯方周 丁壮[2] 

机构地区：[1]辽宁医学院畜牧兽医学院,辽宁锦州121001 [2]吉林大学动物医学院,吉林长春130062

出　　处：《畜牧与兽医》2015年第2期24-29,共6页Animal Husbandry & Veterinary Medicine

基　　金：辽宁省教育厅科研项目(L2014327)

摘　　要：为了研究欧洲型猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)辽宁分离株的遗传变异情况及分子生物学特征,根据欧洲型PRRSV标准毒株LV设计15对引物,对LNEU12毒株全基因分段进行扩增并测序,将测序结果依次拼接得到病毒的全基因,然后进行序列的比对分析。结果表明:LNEU12毒株基因全长为15 398 nt(不含3'尾端序列),与LV毒株同源性为90.1%,其中NSP2和ORF3/4重叠区域缺失21 nt,ORF6、ORF7较为保守。根据基因变异特点和遗传进化亲缘关系推测LNEU12辽宁分离株为欧洲型PRRSV变异毒株。This study was conducted to investigate genetic variation and molecular biological characteristics of European genotype porcine reproductive and respiratory syndrome virus（ EU-PRRSV） isolated from Liaoning Province. Fifteen pairs of oligonucleotide primers were designed based on European prototype LV,and used to amplify genome of LNEU12 isolated from Liaoning Province. The whole-genome sequence was obtained by splicing of correct sequencing and aligned with other strains. The results showed that the whole genome of LXEU12 is15 398 nt,not including poly（ A） sequences. Genetic analyses demonstrated that LXEU12 exhibits 90. 1% with PRRSV strains LV. LXEU12 has 21 nt discontinuous deletions in the nonstructural protein 2 region and the overlapping regions of ORF3 and ORF4 regions,but ORF6 and ORF7 are more conservative. According to the sequence variation characteristics and genetic evolution relationship,we speculate that LNEU12 isolated from Liaoning Province is European genotype PRRSV variant strain.

关 键 词：猪繁殖与呼吸综合征病毒 欧洲型 全基因测序 遗传进化分析 辽宁省 

分 类 号：S852.65[农业科学—基础兽医学]