细菌脂多糖作用下角膜上皮细胞Toll样受体2、4动态变化研究  

Dynamic changes in expression of Toll like receptor on cornea epithelial cells induced by lipopolysaccharide

作　　者：朱海峰[1] 王亮[1] 

机构地区：[1]西安市第一医院,西安710002

出　　处：《陕西医学杂志》2015年第3期276-278,共3页Shaanxi Medical Journal

摘　　要：目的:观察角膜上皮细胞在细菌脂多糖作用后不同时间Toll样受体(TLR)2、4表达的动态变化,初步明确TLR在角膜细菌感染和保持免疫稳态中的作用。方法:培养永生化人角膜上皮细胞系,分别用10ng/ml和1μg/ml的细菌脂多糖(LPS)刺激,作用2h、4h、8h后检测TLR2、4的表达,4h、8h、12h后检测细胞分泌IL-6能力。结果:不同浓度LPS刺激后,细胞TLR2、4的mRNA表达水平在不同时点均明显增高,但随着刺激时间延长,细胞表面蛋白表达和IL-6的分泌水平则呈现先升后降的趋势。结论:人角膜上皮细胞(HCE)能够表达功能性的TLR2、4,并且在接受刺激后,TLR蛋白表达和炎症因子分泌具有负反馈抑制。Objective：To evaluate the dynamic changes in expression of Toll like receptor on cornea epithelial cells induced by lipopolysaccharide and illustrate the effect of TLR in the bacterial infection and Immune homeostasis.Methods：Immortalized human corneal epithelial cell was treated by 10ng/ml and 1μg/ml lipopolysaccharide.The expression of TLR2 and 4were detected with qRT-PCR from mRNA level and FACS from protein level,at 2,4,and 8hours after stimulation.The IL-6level was examined by ELISA at 4,8,and 12 hours after stimulation.Results：After stimulation with LPS,cells elicited an increase in the content of TLR2 and 4specific mRNA at each time point.The cell surface expression of TLR and IL-6secretion increased at early stage but decreased at late stage of LPS exposure.Conclusion：TLR 2and 4cell surface expression in HCE cell can recognize the stimulation of LPS,and their expression pattern constitutes a negative-feedback loop that is important in the bacterial infection and Immune homeostasis environment in the human cornea.

关 键 词：@Toll样受体 上皮角膜 白细胞介素-6/代谢 脂多糖类/代谢 免疫调节 

分 类 号：R392.3[医药卫生—免疫学]