电针对神经病理性疼痛大鼠背根神经节及脊髓P2X3受体的影响  

THE EFFECT OF ELECTROACUPUNCTURE ON THE EXPRESSION OF P2X3 RECEPTOR IN DORSAL ROOT GANGLION AND SPINAL CORD IN RATS WITH NEUROPATHIC PAIN

作　　者：王万胜[1] 屠文展[1] 奚海艳 蒋松鹤[1] 

机构地区：[1]温州医科大学附属第二医院&育英儿童医院康复中心,温州325027 [2]滨州医学院附属医院妇科,滨州256603

出　　处：《中国疼痛医学杂志》2015年第2期97-101,共5页Chinese Journal of Pain Medicine

基　　金：国家自然科学基金(30901924)

摘　　要：目的：探究电针对坐骨神经挤压损伤（chronic constriction injury,CCI）所致神经病理性疼痛大鼠坐骨神经节（DRG）及脊髓P2X3受体的影响并比较同侧电针与对侧电针的差异。方法：选取40只成年雄性SD大鼠,随机分为假模组、模型对照组、对侧电针组、同侧电针组。电针组于造模后第7天给予电针治疗,连续电针7天。所有动物在造模前（第0天）及造模后3、7、10、14天测量机械缩足阈值（MWT）和热缩足反射潜伏期（TWL）。造模14天后运用免疫荧光技术检测各组大鼠L4~6DRG细胞及L4~5脊髓P2X3受体表达情况。结果：CCI造模后大鼠MWT值和TWL值出现明显降低,电针治疗后,MWT值和TWL值均有不同程度的提高（P〈0.01）,且同侧电针组与对侧电针组无明显差异。CCI损伤后DRG及脊髓P2X3表达上调,对侧电针组和同侧电针组蛋白阳性神经元总数少于模型对照组（P〈0.01）。结论：电针可以减轻外周神经损伤后引起的痛觉过敏,电针的镇痛作用可能是通过降低DRG及脊髓的P2X3受体表达来实现的,并且对侧电针与同侧电针有类似的镇痛作用。Objective： To investigate the effect of electroacupuncture on the expression of P2X3 receptor in dorsal root ganglion（DRG） and spinal cord in rats with neuropathic pain caused by chronic constriction injury（CCI） of sciatic nerve（CCI）. Methods： Forty Sprague-Dawley rats were divided into four groups, including sham control, CCI, CCI plus contralateral EA, and CCI plus ipsilateral EA. EA treatment was given for 7 days, daily from the day of operation. The mechanical withdrawal threshold（MWT）and thethermal withdrawal latency（TWL）were detected on the day of operation, and day 3, 7, 10, and 14 after CCI operation. At the day 14, the spinal cord tissues were taken and expression of P2X3 receptor examined by immunofluorescence staining. Results： A significant decrease of MWT and TWL was observed after CCI operation, the MWT and TWL were increased after EA treatment（P〈0.01）, while no significant difference was found between the two EA groups. The expression of P2X3 in DRG and spinal cord was increased after CCI operation, numbers of P2X3-positive cells in the two EA groups was less than that in CCI group（P〈0.01）. Conclusion： EA may reduce hyperalgesia caused by CCI in jury.The analgesic effect of EA might be achieved by decreasing the expression of P2X3 in DRG and spinal cord.

关 键 词：电针 神经病理性疼痛 背根神经节 脊髓 P2X3受体 

分 类 号：R245[医药卫生—针灸推拿学]