5-HT_7受体活化对中脑导水管周围灰质ATP诱发电流的影响  

THE EFFECT OF 5-HT_7 RECEPTOR ACTIVATION ON ATP-INDUCED CURRENT IN RATS PERIAQUEDUCTAL GRAY NEURONS

作　　者：肖智[1] 李尤艳 

机构地区：[1]遵义医学院医学与生物学研究中心 [2]遵义医学院研究生学院,遵义563003

出　　处：《中国疼痛医学杂志》2015年第2期102-106,共5页Chinese Journal of Pain Medicine

基　　金：贵州省科学技术基金项目[黔科合J字[2011]2123号]

摘　　要：目的:探讨中脑导水管周围灰质(periaqueductal gray,PAG)神经元5-HT7受体活化对α,β-me ATP诱发内向电流的影响。方法:取SD大鼠乳鼠PAG组织进行原代神经元细胞培养,运用全细胞膜片钳技术观察PAG神经元α,β-me ATP激活电流以及5-HT7受体活化对PAG神经元α,β-me ATP激活电流的影响。结果:α,β-me ATP能够引起PAG神经元产生3种内向电流,即快反应电流、慢反应电流和混合电流。其中快反应内向电流能够被P2X3受体特异性阻断剂A-317491阻断;5-HT7受体特异性激动剂AS-19对α,β-me ATP激活快电流的增大效应呈浓度依赖性;5-HT7受体特异性阻断剂SB-269970能够翻转AS-19对α,β-me ATP激活快电流的增大效应。结论:PAG神经元上5-HT7受体可与P2X3受体在功能上产生协同作用,进而促进机体内源性镇痛系统的功能,产生镇痛作用。Objective： To explore the effect of 5-HT7 receptor activation on ATP-induced current amplitudes in primary cultured periaqueductal gray（PAG） neurons. Methods： Using primary cultured PAG neuronal cells of newborn Sprague-Dawley（SD） rats, to observe the α,β-me ATP induced current amplitude and the effects of 5-HT7 receptor activation on this α,β-me ATP induced inward current in cultured PAG neurons with whole-cell patch clamp recording technique. Results： α,β-me ATP could produce three types of inward currents on PAG neurons（transient, sustained and biphasic）. The transient current and the transient component of the biphasic current were observed. The transient current was blocked by A-317491, a selective P2X3/P2X2/3 receptor antagonist; AS-19, a 5-HT7 receptor selective agonist, could increase the amplitude of α,β-me ATP induced currents in a dose-dependent manner but reversed by pretreatment with SB-269970, a specific antagonist of 5-HT7 receptor. Conclusion： 5-HT7 receptor activation in the PAG neurons can produce a synergistic effect with P2X3 receptor. The activation of 5-HT7 receptor on PAG neurons produces an analgesic effect via P2X3 signaling pathway and promote endogenous analgesic system function.

关 键 词：5-HT7受体 P2X3受体 中脑导水管周围灰质 膜片钳 

分 类 号：R338[医药卫生—人体生理学]