当归芍药散对抗结核药物致大鼠肝损伤的保护作用及其机制研究  

Protection of Danggui Shaoyao Powder water extracts on hepatic injury induced by antituberculosis drugs in rats and its mechanism

作　　者：文高艳[1] 曾谊[1] 冯枭[1] 何花[1] 宋梅梅[1] 朱珠[1] 

机构地区：[1]南京市胸科医院,江苏南京210000

出　　处：《现代药物与临床》2015年第2期120-125,共6页Drugs & Clinic

基　　金：南京市医学科技发展专项资金资助一般性课题(YKK12131)

摘　　要：目的探讨当归芍药散水提取物对抗结核药物肝损伤的保护作用及其机制。方法将60只SD大鼠随机分为6组,即对照组、模型组、硫普罗宁组(60 mg/kg)和当归芍药散水提取物低、中、高剂量(8、16、32 g/kg)组,给药8周。分别测定大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平及血清和肝脏超氧化物歧化酶(SOD)、丙二醛(MDA)水平;采用RT-PCR方法测定大鼠肝脏肿瘤坏死因子-α(TNF-α)m RNA表达水平;利用Western Blotting法测定大鼠肝脏TNF-α蛋白表达水平。结果与模型组比较,当归芍药散水提取物中、高剂量组均显著降低血清ALT、AST水平,上调血清和肝脏SOD活性,下调血清和肝脏MDA水平,并降低肝脏TNF-αm RNA和蛋白表达水平。结论当归芍药散水提取物对抗结核药物肝损伤的保护作用机制可能与抑制TNF-α表达和脂质过氧化有关。Objective To investigate the protection of Danggui Shaoyao Powder water extracts on the hepatic injury induced by antituberculosis drugs in rats. Methods Sixty rats were divided randomly into six groups： Normal group, model group, tiopronin group（60 mg/kg）, and Danggui Shaoyao Powder water extracts（8, 16, and 32 g/kg） groups. All groups were treated for 8 weeks. The levels of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） in serum, as well as the superoxide dismutase（SOD） and malondialdehyde（MDA） in serum and liver were determined. Expression of hepatic tumor necrosis factor-α（TNF-α） m RNA was analyzed by RT-PCR method and its protein levels were assayed by Western Blotting method. Results Compared with model group, Danggui Shaoyao Powder water extracts at medium and high doses significantly reduced serum ALT and AST levels, elevated serum and liver SOD activities, down-regulated serum and liver MDA levels and reduced liver TNF-α m RNA and protein levels. Conclusion Danggui Shaoyao Powder water extracts has protective effects on the hepatic injury induced by antituberculosis drugs in rats which may be related to its inhibition of TNF-α and lipid peroxidation.

关 键 词：当归芍药散 水提取物 肝损伤 TNF-α 脂质过氧化 保护作用 

分 类 号：R285.5[医药卫生—中药学]