肾康灵调控系膜细胞炎性反应因子的机制研究  

Study on Mechanism of Shenkangling Regulating Inflammatory Factor of Mesangial Cells

作　　者：艾斯[1,2,3] 郑健[1,2,3] 林青[2,3] 邱彩霞[1,2] 林雄[2] 宋艳芳[2] 

机构地区：[1]福建中医药大学,福州350122 [2]福建中医药大学附属人民医院,福州350004 [3]福建省中西医结合肾脏病重点实验室,福州350004

出　　处：《世界中医药》2015年第2期236-242,共7页World Chinese Medicine

基　　金：福建省自然科学基金面上项目(编号:2011J01199);国家自然科学基金青年基金项目(编号:81202835);福建省卫生厅青年基金项目(编号:2012-1-30)

摘　　要：目的:观察氧化低密度脂蛋白(Oxidized Low-Density Lipoprotein,ox-LDL)对系膜细胞(Mesangial Cells,MCs)分泌炎性反应递质功能的影响,并从细胞分子生物学水平阐明肾康灵的作用机制。方法:采用肾康灵干预增殖的系膜细胞,并利用分子生物学技术检测CXCL16、CD36、IFN-γ、IL6、TNF-α含量或基因水平。结果:利用ox-LDL诱导大鼠系膜细胞增殖并加入CXCR6受体后,CXCL16、CD36、IFN-γ、IL6、TNF-α的含量或基因水平显著升高,肾康灵呈浓度依赖性降低其表达水平。结论:ox-LDL诱导系膜细胞增殖时通过CXCR6介导,可促进CXCL16、CD36、IFN-γ、IL6、TNF-α等炎性反应递质的释放,中药肾康灵可通过抑制炎性反应递质的释放,保护系膜细胞的功能。Objective： To clarify the mechanism of Shenkangling on mesangial cells and to observe oxidized low density lipoprotein（ ox-LDL） induced Mesangial Cells（ MCs） secretion of inflammatory cytokine. Methods： MCs were treated with Shenkangling,and the levels of the inflammatory cytokines,such as CXCL16,CD36,IFN-γ,IL6,TNF-αby ELISA,Real Time-PCR and Western blot were detected. Results： The levels of CXCL16,CD36,IFN-γ,IL6,TNF-α were significantly increased after CXCR6 joined and oxLDL induced mesangial cell proliferation,then all levels were significantly reduced by Shenkangling in a concentration dependent manner. Conclusion： ox-LDL may promote the release of inflammatory mediators such as in mesangial cells,and CXCR6 may mediate this path. Shenkangling has effects for ameliorating ox-LDL-induced MC proliferation and inflammatory reactions.

关 键 词：肾康灵 系膜细胞 氧化低密度脂蛋白 CXCL16 CD36 

分 类 号：R692.602[医药卫生—泌尿科学]