针刺对大鼠脑缺血再灌注损伤后不同时间点血清SOD和MDA表达的影响!  

Expression of SOD and MDA in the Serum of Rats Subjected to Cerebral Ischemic-reperfusion Treated by Acupuncture

作　　者：王富明[1] 张亚敏[1] 孙华[1] 徐虹[1] 陈素辉[1] 杨杨[1] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院,北京100730

出　　处：《针灸临床杂志》2015年第2期62-65,共4页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金项目;编号:81273850

摘　　要：目的:探讨针刺百会、足三里穴对脑缺血再灌注损伤大鼠不同时间点血清SOD、MDA表达的干预作用。方法:将SD大鼠随机分为假手术组、模型组、电针组和药物组。后3组制作脑缺血再灌注模型,根据再灌注时间分为1天、3天、5天和7天组。电针组针刺百会、左足三里,日1次/20 min,药物组腹腔注射依达拉奉3 mg/kg,日1次。黄嘌呤氧化酶法测血清SOD活力,TBA法测血清MDA含量。结果:模型组SOD活性先降低,后升高,低于同时间点的电针组和药物组;MDA含量先升高,后降低,高于同时间点的电针组和药物组;电针组SOD活性低于同时间点的药物组,MDA含量高于同时间点的药物组。结论:针刺大鼠百会、足三里穴可能通过上调SOD、下调MDA表达干预脑缺血再灌注损伤后氧化应激和脂质过氧化过程。Objective：To explore the effect of acupuncture points at Baihui（GV20） and Zusanli（ST36） on the expression of SOD and MDA in serum of rats subjected to cerebral ischemic-reperfusion injury（ CIRI） .Meth-ods：Adult Sprague-Dawley rats were randomly divided into following groups：the sham （ S） group, the middle cerebral artery occlusion （ MCAO ） group, the electro -acupuncture （ EA ） group and the edaravone （ ED ） group.Rats in MCAO, EA, and ED groups subjected to 2 h MCAO followed by 1, 3, 5, 7 d of reperfusion. The rats in EA group received electro-acupuncture treatment by needling at GV20 and left ST36;rats in EA and ED groups received electroacupuncture, or edaravone, respectively, once a day.Xanthine oxidase method was used to detect serum SOD activity and TBA method was used to detect the serum MDA content.Results：The trend of expression levels of SOD in serum of rats in MCAO group was increased,but then decreased.The levels of SOD in MCAO group were significantly lower than those in EA and ED groups.The trend of the ex-pression levels of MDA in serum of the rats in MCAO group was decreased,but then increased.The levels of MDA in MCAO group were significantly higher than those in EA and ED groups.The levels of SOD in EA group was significantly lower than those in ED group,and the levels of MDA in EA group was significantly higher than that in ED group.Conclusion：Acupuncturing at GV20 and ST36 has a protective effect on cerebral ischemia reperfusion injury rats.This effect might be via oxidative stress and lipid peroxidation by increasing the levels of SOD and decreasing the levels of MDA.

关 键 词：针刺 百会 足三里 脑缺血再灌注损伤 超氧化物歧化酶 丙二醛 

分 类 号：R246.6[医药卫生—针灸推拿学]