木荷组织培养技术研究  

Tissue Culture Technique of Schima superba

作　　者：陈碧华[1,2,3] 张娟[1,2] 江斌 邓永生 肖玉梅 张翠 

机构地区：[1]福建省林业科学研究院,福州350012 [2]国家林业局南方山地用材林培育重点实验室,福州350012 [3]福建省清流县林业局,三明365300

出　　处：《湖北林业科技》2015年第1期16-19,共4页Hubei Forestry Science and Technology

基　　金：福建省属公益类科研院所基本科研专项(闽林研〔2012〕25号);福建省林业厅科研项目"木荷优良单株选择与组培技术研究"(闽林科〔2012〕3号)

摘　　要：对木荷外植体取材母株提前1周喷杀虫剂和杀菌剂,外植体污染率明显降低。加入Vc可以减少外植体褐化,并采用全暗培养。选择MS+BA0.5 mg·L-1+NAA0.1 mg·L-1+Vc 5mg·L-1作为木荷最佳外植体诱导培养基,MS+BA0.5 mg·L-1+NAA0.1 mg·L-1+Vc 5 mg·L-1+B25 mg·L-1为木荷最佳增殖培养基,1/4MS+IBA0.25 mg·L-1作为木荷最佳生根培养基。The contamination rate of the explants of Schima superba during the process of tissue culture was decreased evidently by pre-treating the stock plants using the pesticides and fungicides before one week in advance.The browning rate of the explants was decreased by adding the Vitamine C and being culured in dark.MS＋BA0.5 mg·L^-1＋NAA0.1mg·L^-1＋Vc 5 mg·L^-1 was adopted as the optimal explant induction medium,MS＋ BA0.5 mg·L^-1＋NAA0.1 mg·L^-1＋Vc 5 mg·L^-1＋ B25 mg·L^-1 was adopted as the optimal proliferation medium,and 1/4MS＋IBA0.25 mg·L^-1 was adopted as the optimal rooting medium.

关 键 词：木荷 组织培养 外植体诱导培养基 增殖培养基 生根培养基 

分 类 号：Q813.12[生物学—生物工程]