抗丙型肝炎病毒抗体胶体金免疫层析法的建立  

The establishment of a colloidal gold-based immunochromatographic assay for detection of hepatitis C virus antibody

作　　者：李静芳[1,2] 齐红圆 王云龙 李玉林 王继创 程蕾 

机构地区：[1]新乡医学院,河南新乡453003 [2]河南省生物工程技术研究中心,河南郑州4500012

出　　处：《现代预防医学》2015年第6期1078-1080,1107,共4页Modern Preventive Medicine

基　　金：河南省科技攻关;郑州市科技创新领军人才基金项目(131PLJRC666)

摘　　要：目的研制一种检测丙型肝炎病毒（Hepatitis C Virus,HCV）血清抗体的胶体金双抗原夹心免疫层析检测法。方法将丙肝重组抗原与胶体金连接形成免疫金,质控线与检测线分别包被HCV单抗与丙肝重组抗原,在样品垫上滴加10μl血清和90μl PBS溶液展开。通过加入6种非丙型肝炎阳性血清测试试纸条的特异性;将试纸条用国家血清盘质控品进行灵敏度测试;放置3种温度环境下10周,每周进行2次抽检,进行稳定性测试。结果本检测方法特异性、灵敏度且稳定性均较好,并可在10~15 min内判读结果,与酶联免疫法检测相比,检验符合率能达到较高水平。结论该法能够快速检测人血清中抗HCV抗体。Objective The study was conducted to develop a colloidal gold-based immunochromatographic assay for detection of hepatitis C virus（HCV） antibody in human serum. Methods Recombinant hepatitis C antigen was linked to colloidal gold to form immune gold. Quality control and detection strings were covered with HCV monoclonal antibody and recombinant hepatitis C antigen, respectively. 10 μl of serum and 90 μl of PBS were spotted on the sample pads. Specificity of the test strip was tested by adding 6 types of non-hepatitis C-positive serum; sensitivity was tested by using the QC samples in the national serum panel; and stability was tested by placing the strips at three different temperatures for 10 weeks and sampling inspected twice a week. Results The method was good in specificity, sensitivity, and stability, and gave readable results within 10-15 min. The rate of compliance with ELISA results was high. Conclusion The method is capable of fast detection of anti-HCV antibody in human serum.

关 键 词：丙型肝炎病毒 双抗原夹心法 胶体金 

分 类 号：R319[医药卫生—基础医学]