七叶皂苷A的大鼠血浆蛋白结合率研究  

Study on plasma protein binding rate of escin A in rat' plasma

作　　者：吴秀君[1,2] 崔向勇[2] 高峰[2] 关鑫[2] 何群[2] 顾景凯[2] 

机构地区：[1]辽宁中医药大学附属医院中药临床药代动力学实验室,沈阳110032 [2]吉林大学生命科学学院药物代谢研究中心,长春130021

出　　处：《中国临床药理学杂志》2015年第5期358-361,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30772613);"十一五"国家科技重大专项基金资助项目(2009ZX09304-003)

摘　　要：目的研究七叶皂苷A的大鼠血浆蛋白结合率。方法在体外用平衡透析法模拟七叶皂苷A体内与血浆蛋白结合的过程,用高效液相色谱-质谱联用(HPLC-MS/MS)法测定低、中、高3个浓度的七叶皂苷A及其转化产物七叶皂苷C在透析袋内血浆中和透析袋外缓冲液中的浓度,计算七叶皂苷A的血浆蛋白结合率。结果低、中、高3个浓度的七叶皂苷A在体外与大鼠的平均血浆蛋白结合率分别为95.8%,96.6%和95.9%,3个浓度之间的血浆蛋白结合率比较差异无统计学意义(P>0.05)。结论七叶皂苷A与大鼠血浆蛋白高度结合。Objective To study the plasma protein binding rate of escin A in rats＇plasma. Methods Under three different concentrations, this paper used equilibrium dialysis method to imitate the binding process be- tween escin A and the plasma protein, and determined the concentration of escin A and escin C （a transformation product of escin A） in and out of the dialysis membrane using high performance liquid chromatography - tandem mass spectrometry （LC -MS/MS） method and calculated the plasma protein binding rate of escin A. Results The plasma protein binding rate of escin A in rats was 95.8% , 96. 6% and 95.9% at low, middle and high concentration, respectively. There was no significant difference among three concentrations. Conclusion Escin A was highly bound to plasma proteins in rats.

关 键 词：七叶皂苷A 血浆蛋白结合率 平衡透析法 

分 类 号：R285.5[医药卫生—中药学] R969.1[医药卫生—中医学]