降糖宁胶囊对HepG2细胞糖代谢作用及其作用机制的研究  

Glucometabolism of Jiangtangning Capsules on HepG2 cells and its mechanism

作　　者：赵清辉[1] 王琳[1] 陈慧芳[1] 谢欣梅[1] 庞晓斌[1] 

机构地区：[1]河南大学药物研究所,河南开封475004

出　　处：《中成药》2015年第3期483-487,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金(81273652)

摘　　要：目的观察降糖宁胶囊(人参、山药、石膏、知母等)对Hep G2细胞糖代谢的影响,并探讨其降糖机制。方法体外培养人肝癌Hep G2细胞,MTT法检测降糖宁对细胞活力的影响;HE染色法观察降糖宁对细胞形态的影响;采用葡萄糖氧化酶法检测降糖宁对细胞糖消耗的影响;Western-blot检测磷酸化腺苷酸活化蛋白激酶(AMPK)及人胰岛素受体底物(IRS1)表达量。结果降糖宁胶囊对Hep G2细胞的活力和形态无明显影响,可明显促进Hep G2细胞对葡萄糖的消耗,能促进AMPKβ1/2、IRS1蛋白表达量。结论降糖宁胶囊的降糖机制可能涉及对胰岛素受体信号通路及AMPK信号通路的影响。AIM To explore the effect of Jiangtangning Capsules（ Ginseng Radix et Rhizoma,Dioscoreae Rhizoma,Gypsum Fibrosum,Anemarrhenae Rhizoma,etc.） on glucose metabolism of HepG2 cells in vitro and its mechanism.METHODS MTT assay was used to measure the viability of the HepG2 cells.HE staining was used for the observation of the morphological changes in HepG2 cells under the influence of Jiangtangning Capsules and glucose consumption was detected by oxidation enzyme method.The AMP-activated protein kinase（ AMPK） and IRS-activated protein kinase（ IRS1） were examined by Western-blot.RESULTS Jiangtangning Capsules could obviously promote the glucose consumption,AMPKβ1 /2,and the IRS1 protein expression of the HepG2 cells without affecting its viability or causing morphological change.CONCLUSION The hypoglycemic mechanism of Jiangtangning Capsules may be associated with insulin receptor signaling pathways and AMPK pathway.

关 键 词：降糖宁胶囊 HEP G2 糖代谢 AMPK IRS1 

分 类 号：R285.5[医药卫生—中药学]