甜菜碱调控RhoA/ROCK信号通路抑制大鼠急性心肌缺血损伤  

Betaine protects against isoproterenol-induced acute myocardial ischemic injury through the regulation of Rho A / ROCK pathway

作　　者：袁娅妮 毛福英[1] 刘俊梅[1] 买淑霞 王洋 戴贵东[3] 郑萍[1,4] 

机构地区：[1]宁夏医科大学药学院药理学系,宁夏银川750004 [2]银川市口腔医院,宁夏银川750004 [3]凯里学院化学与材料工程学院制药工程教研室,贵州凯里556011 [4]宁夏回药现代化工程技术研究中心,宁夏银川750004

出　　处：《中成药》2015年第3期492-496,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(81160403)

摘　　要：目的探讨从枸杞中分离得到的甜菜碱对异丙肾上腺素(ISO)诱导的大鼠急性心肌缺血损伤的保护作用机制。方法大鼠分别灌胃给予不同剂量甜菜碱(100、200和400 mg/kg)共40 d,于第39天和第40天皮下注射ISO85 mg/(kg·d)诱发大鼠急性心肌缺血。取大鼠心肌组织进行病理学观察,ELISA法测定血清TGF-β1与ET-1的水平,并采用Western-blot法测定相关蛋白的表达。结果与模型组比较,甜菜碱(100、200和400 mg/kg)明显改善心肌组织坏死,炎细胞浸润及间质水肿。甜菜碱(100、200、400 mg/kg)可明显降低血清TGF-β1及ET-1的水平(P<0.01),抑制心肌组织Rho A、ROCK1、ROCK2蛋白表达的升高(P<0.01)。结论甜菜碱可通过调控Rho A/ROCK信号通路保护ISO诱导的大鼠急性心肌缺血损伤。AIM To investigate the cardioprotective effects of betaine extracted from Lycium barbarum L.on regulation of Rho A / ROCK pathway in isoproterenol-induced acute myocardial ischemia.METHODS Betaine（ 100,200 and 400 mg /kg） was administered orally once a day for forty days,and acute myocardial injury was induced by subcutaneous injection of isoproterenol（ 85 mg / kg） in rats on the 39 th and 40 th day.Myocardial injury was examined by histopathological evaluation.Serum levels of transforming growth factor（ TGF）-β1 and endothelin（ ET）-1 were analyzed by ELISA.In addition,the protein expression of Rho A、ROCK1and ROCK2 were measured by Western-blot.RESULTS Oral administration of betaine（ 100,200 and 400 mg / kg） attenuated myocardial necrosis,interstitial edema,and inflammatory cell infiltration.Moreover,the treatment with betaine decreased isoproterenol-induced elevated serum levels of TGF-β1 and ET-1（ P〈0.01） as well as increased protein expression of Rho A,ROCK1 and ROCK2（ P〈0.01）.CONCLUSION The present study has demonstrated that betaine protects against isoproterenol-induced myocardial ischemia via down-regulating Rho A / ROCK pathway.

关 键 词：甜菜碱 异丙肾上腺素 急性心肌缺血 TGF-β1 ET-1 RHO A/ROCK信号通路 

分 类 号：R285.5[医药卫生—中药学]