RP-HPLC法同时测定线纹香茶菜不同生长部位中三萜酸成分  

Simultaneous determination of five triterpenic acids in different parts of Rabdosia lophanthoides by RP-HPLC

作　　者：邹盛勤[1] 姜琼[1] 

机构地区：[1]江西省天然药物活性成分研究重点实验室,宜春学院化学与生物工程学院,江西宜春336000

出　　处：《中成药》2015年第3期570-573,共4页Chinese Traditional Patent Medicine

基　　金：国家“863”计划重点资助项目(2002AA2Z3217);江西省科技计划项目(2007BG07200)

摘　　要：目的建立反相高效液相色谱法（RP-HPLC）同时测定线纹香茶菜全草、根、茎和叶中坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸5种三萜酸的含有量。方法线纹香茶菜95%乙醇提取液的分析在Kromasil C18色谱柱（4.6mm×250mm,5μm）上进行,流动相为甲醇-2.0%乙酸水溶液（88∶12,V/V）,体积流量1.0mL/min,检测波长210nm,柱温25℃。结果坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸进样量分别在0.552-41.4 mg/L（r=0.999 5）,0.540-40.5 mg/L（r=0.999 6）,0.824-61.8mg/L（r=0.999 9）,2.444-183.3mg/L（r=0.999 7）和3.172-237.9mg/L（r=0.999 7）范围内线性关系良好,加样回收率分别为98.1%、98.0%、96.3%、99.7%、99.0%。RSD（n=6）均小于2.2%。结论线纹香茶菜样品不同部位中,5种三萜酸类成分含有量高低依次为叶〉全草〉茎〉根。AIM To establish the method for simultaneously determining pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid in total grass,root,stem,and leaf of Rabdosia lophanthoides.METHODS The separation was achieved on a Kromasil C18column（ 4.6 mm × 250 mm,5 μm） at 25 ℃ using methanol-2.0% CH3 COOH solution（ 88 ∶ 12,V/V） as the mobile phase at a flow rate of 1.0 m L / min.The detection wavelength was set at 210 nm.RESULTS Linearities of pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid were in the ranges of 0.552- 41.4 mg/L（ r = 0.999 5）,0.540- 40.5 mg/L（ r =0.999 6）,0.824- 61.8 mg/L（ r = 0.999 9）,2.444- 183.3 mg/L（ r = 0.999 7） and 3.172- 237.9 mg/L（ r = 0.999 7）,respectively.The average recoveries were 98.1%,98.0%,96.3%,99.7% and 99.0% with RSD（ n = 6） less than 2.2%.CONCLUSION The five triterpenic acids from R.Lophanthoides distributed from high to low are leaf 〉total grass 〉stem 〉root in turn.

关 键 词：线纹香茶菜 三萜酸 坡模酸 山楂酸 科罗索酸 齐墩果酸 熊果酸 

分 类 号：R284.1[医药卫生—中药学]