盐胁迫下水稻苗高和分蘖数的发育动态QTL分析  

Dynamic QTL Analysis of Rice Seedling Height and Tiller Number Under Salt Stress

作　　者：孙健[1] 王敬国[1] 刘化龙[1] 谢冬微[2] 郑洪亮[1] 赵宏伟[1] 邹德堂[1] 栾非时[3] 

机构地区：[1]东北农业大学农学院水稻研究所,黑龙江哈尔滨150030 [2]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150030 [3]东北农业大学园艺学院,黑龙江哈尔滨150030

出　　处：《核农学报》2015年第2期235-243,239-243,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家科技支撑计划(2013BAD20B04-1S;2011BAD35B02-01;2011BAD16B11)

摘　　要：为了检测盐胁迫下水稻苗高和分蘖数的发育动态QTL,以粳稻品种东农425和长白10为亲本衍生的F2:3群体为试验材料,构建了包含123个SSR标记,全长为1 616.53 c M,平均图距为13.14 c M的遗传连锁图谱。以浓度为6 ds·m-1的Na Cl水溶液进行大田生育期灌溉,正常水灌溉为对照,对盐胁迫下水稻的苗高和分蘖数进行发育动态QTL分析。分别利用完备区间作图法和混合线性模型的QTL定位方法,联合盐胁迫与正常条件下6个发育时期苗高和分蘖数的表型数据,共检测到6个控制盐胁迫下水稻苗高和3个控制分蘖数的加性QTL、4个控制正常条件下苗高和5个控制分蘖数的加性QTL、盐胁迫和正常条件联合下的6个控制苗高和4个控制分蘖数的加性QTL,以及3对控制苗高和1对控制分蘖数的上位性QTL。加性QTL q SH1在t3、t4和t2/t1时期分别用非条件和条件方法检测到,加性QTL q TN8-2在t2、t3、t4和t5时期被连续用非条件方法检测到,在t3/t2时期用条件方法被检测到。分别检测到4个控制苗高和2个控制分蘖数的加性QTL与盐胁迫环境存在互作效应,控制苗高的3对上位性QTL和控制分蘖数的1对上位性QTL均与盐胁迫环境发生互作。本研究旨在检测不同发育时期控制盐胁迫下水稻苗高和分蘖数的QTL,并分析与盐环境的互作效应,为解析苗高和分蘖数在盐胁迫下的发育遗传特点和水稻耐盐QTL分子标记辅助育种提供理论依据。In order to detect the dynamic QTLS of rice seedling height population derived from two japonica varieties of ＇Dongnong＇ 425 x and tiller number under salt stress, a F2,3 ＇Changbai＇ 10 was used as experimental materials, and a genetic linkage map was constructed containing 123 SSR markers, 1616. 53 cM full-length and 13.14 cM average distance. A NaC1 solution （6 dS·m -1 ） was used to irrigate the field and the control was irrigated with water to analyze the dynamic QTL of rice seedling height and tiller number under salt stress. Using QTL mapping method of inclusive composite interval mapping and mixed linear model, a total of six additive QTLs controlling seedling height and three controlling tiller number under salt stress, four additive QTLs controlling seedling height and five controlling tiller number under normal condition, six additive QTLs controlling seedling height and four controlling tiller number under the union of salt stress and normal condition, and three pairs of epistatic QTLs controlling seedling height and one controlling tiller number were detected uniting the phenotypic data of seedling height and tiller number during six developmental stages under salt stress and normal condition. Additive QTL qSH1 was detected at t3, t4 and t2/tl stages using unconditional and conditional QTL mapping methods, respectively. Additive QTL qTN8-2 was detected continuously at t2, t3, t4 and t5 stages using unconditional method, and was detected at t3/t2 stage using conditional method. Four additive QTLs controlling seedling height and two controlling tiller number was detected having interaction effects with the salt stress environment. Three pairs of epistatic QTLs controlling seedling height and one pair of controlling tiller number were all interacted with the salt stress environment. The objective of this study was to detect QTLs controlling the seedling height and tiller number of rice under salt stress in various developmental stages, and to analyze the interaction effects with the salt stres

关 键 词：水稻 盐胁迫 苗高 分蘖数 不同发育时期 QTL与环境互作 

分 类 号：S511.22[农业科学—作物学]