盐碱胁迫对甜菜氮代谢相关酶活性及产量和含糖率的影响  

Effects of Saline-alkali Stress on Nitrogen Metabolism Activity and Root Yield and Sugar Content of Suger Beet

作　　者：刘洋[1] 李彩凤[1] 洪鑫[1] 徐颖[1] 郭剑[1] 陈明[1] 于洋[1] 王玉波[1] 马凤鸣[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《核农学报》2015年第2期397-404,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金项目(31171493)

摘　　要：利用KWS0143和ACERO为试验材料,比较盐碱(碳酸钠)胁迫对2个甜菜品种氮代谢关键酶活性及产量和含糖率的影响,旨在进一步明确甜菜对盐碱胁迫的适应性。通过桶栽试验,设5个处理,分别是碳酸钠占土壤质量百分比0、0.5%、1%、1.5%和2%,对应的土壤溶液p H值分别为7.14、8.92、9.45、10.19、10.56。研究盐碱胁迫对甜菜叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合酶(GOGAT)活性及产量和含糖率的影响。结果表明,盐碱胁迫使甜菜叶片的硝酸还原酶(NR)、谷氨酰胺合成酶(GS)及谷氨酸合酶(GOGAT)活性均下降,且随着盐碱胁迫程度的增大,下降幅度增大。两品种比较,ACERO的酶活性高于KWS0143,且ACREO的块根产量和含糖率下降的幅度低于KWS0143,ACREO比KWS0143具有较强的耐盐碱能力。该研究为进一步提高盐碱胁迫下甜菜对氮素的吸收利用效率理论依据。In order to further clarify the adaptability of sugar beet to saline-alkali stress, KWS0143 and ACERO were used as test material to compare the influence of saline-alkali （Na2CO3） stress on key enzymes activities of nitrogen metabolism,root yield and sugar content of two varieties. By conducting pot experiment,we set 5 treatments in which the proportion of sodium carbonate in soil quality is set to five levels：0,0. 5%, 1%, 1.5% ,2%, and the corresponding values of the soil solution pH was 7.14,8.92,9.45,10. 19,10. 56 respectively. In this way, we studied the changes of saline-alkali stress on nitrate reductase（NR） ,glutamine synthetase （GS） and glutamate synthase（GOGAT） activity, root yield and sugar content. The results were as follows ： the activitives of nitrate reductase （NR） glutamine synthetase （ GS ） and glutamate synthase （GOGAT） in the leaf of sugar beet under the saline-alkali stress were decreased, and as the degree of saline-alkali stress increases, the decline increases. Comparing the two varieties, the enzymatic activity of ACERO was higher than KWS0143 ,and the drop rate of yield and sugar content of ACERO＇ s root was less than that of KWS0143＇s. Therefore, saline-alkali resistance ability of ACERO was stronger than that of KWS0143. The research provides a theoretical basis for the further improving nitrogan absorption and utilization in saline-alkali stress on suger beet.

关 键 词：甜菜 盐碱胁迫 硝酸还原酶 谷氨酰胺合成酶 谷氨酸合酶 

分 类 号：S566.3[农业科学—作物学]