外源海藻糖对冬小麦低温下胚芽长及幼苗抗寒性的影响  

Effect of Exogenous Trehalose on Germ Length and Seedling Freeze Resistance of Winter Wheat under Cold Stress

作　　者：谢冬微[1,2] 王晓楠[1] 付连双[1] 孙健[1] 李卓夫[1] 郑伟[3] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150030 [3]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007

出　　处：《麦类作物学报》2015年第2期215-223,共9页Journal of Triticeae Crops

基　　金：黑龙江新农村建设科技服务项目;黑龙江高校寒地作物品种改良与生态重点开放实验室基金项目(hzzw-006)

摘　　要：为了解外源海藻糖对小麦抗寒性的影响与作用机理,通过室内盆栽,模拟低温(冷冻)条件,研究外源海藻糖处理下抗寒品种东农冬麦1和弱抗寒品种济麦22的胚芽长、抗寒性相关指标及返青率。结果表明,在4℃条件下,抗寒品种东农冬麦1号在10mmol·L-1海藻糖处理下胚芽生长最快,弱抗寒品种济麦22在50mmol·L-1海藻糖处理下胚芽生长相对较快。在-5^-25℃冷冻处理后,东农冬麦1号和济麦22分别在10mmol·L-1和50mmol·L-1海藻糖处理下,分蘖节相对含水量、脯氨酸含量、可溶性糖含量及返青率相对较高,MDA含量较低。结果说明,外源施加一定浓度的海藻糖可以在一定程度上提高冬小麦对低温(冷冻)的抵抗能力。Low temperature is a kind of abiotic stress factors for plant,which is an important limiting factor for the yield of winter wheat in high latitudes areas.In order to know the effects that exogenous trehalose to the embryo length,indexes of cold resistance and reviving rate of winter wheat,Dongnongdongmai 1（cold resistance）and Jimai 22（weak cold resistance）were disposed with different concentrations of exogenous trehalose and freezed at different low temperature in pots indoor.The results showed that the germ was longest for cold resistance variety Dongnongdongmai 1when trehalose was10mmol·L^-1 under 4℃,but for cold sensitive variety Jimai 22 was 50mmol·L^-1.The seedlings of Dongnongdongmai 1pretreatmented with 10mmol·L^-1 trehalose had higher relative water content,higher contents of proline and soluble sugar,and higher regreen rate,but lower MDA content in crown under freezing treatment,while the seedlings of Jimai 22 pretreatmented by 50 mmol·L^-1 trehalose had higher contents of proline and soluble sugar,and higher regreen rate,but lower MDA content in croun.So the resistance of winter wheat to low temperature（freeze）can be improved by exogenous trehalose at certain concentrations.

关 键 词：海藻糖 低温胁迫 抗寒指标 返青率 冬小麦 

分 类 号：S512.1[农业科学—作物学] S311