38株灵芝子实体抗肿瘤质量的生物评价  

The Bio-assay on Anti-tumor Quality Evaluation of the Fruit Bodies of 38 Ganoderma lucidum Strains

作　　者：余梦瑶[1] 许晓燕[1] 魏巍[1] 江南[1] 邱欣玉 罗霞[1] 

机构地区：[1]四川省中医药科学院,四川成都610041 [2]四川大学生命科学学院,四川成都610041

出　　处：《中国食用菌》2015年第2期47-51,共5页Edible Fungi of China

基　　金：菌类药材研究与开发四川省科技创新团队(2011JTD0021);四川省科技支撑计划项目(2013NZ0029);四川省十二五育种攻关项目(2011NZ0098-12-04);四川省食用菌精深加工研究岗位建设项目[川农业(2009)75号];四川省微生物资源共享平台--农业微生物平台项目;四川省重大科技计划项目(2012NZ0003)

摘　　要：针对38株灵芝子实体样品,开展醇提物抑制He La、SK-OV-3肿瘤细胞增殖活性和三萜含量测定研究,对其抗肿瘤质量进行评价,筛选具有开发抗肿瘤产品潜力的优质菌株。实验结果表明,33号、34号2株灵芝对He La细胞和SK-OV-3细胞均具有较强的杀伤作用,对2株细胞增殖抑制IC50分别为16.55μg·mL^-1、10.2μg·mL^-1和31.9μg·mL^-1、30.64μg·mL^-1,可作为优质资源进行进一步开发。各菌株灵芝子实体的三萜含量在1.372 mg·g^-1～6.184 mg·g^-1之间,不同菌株子实体间三萜含量差异较大,细胞增殖抑制活性与三萜含量间不具有相关性,提示灵芝抗肿瘤原料质量评价宜考虑补充生物质量评价方法。In order to evaluate the anti-tumor quality of the fruit bodies of 38 Ganoderma lucidum strains, the IC50 of proliferative inhibition on He La and SK-OV-3 cells and the triterpene content was measured. The IC50 of two strains, No.33 and No.34,on He La and SK-OV-3 cells was 16.55 μg·mL^-1, 10.2 μg·mL^-1and 31.9 μg·mL^-1, 30.64 μg·mL^-1, respectively, which were identified as the potential materials for anti-tumor products developing. The triterpene content of the Ganoderma lucidum fruit bodies was between 1.372 mg·g^-1 to 6.184 mg·g^-1, and was varied extensively in different strains. Meanwhile, the relationship between the activities of anti-tumor and triterpene content was not found. And it suggested that the bio-assay may be taken into consideration in the further anti-tumor quality evaluation of Ganoderma lucidum.

关 键 词：灵芝 抗肿瘤 三萜 生物评价 

分 类 号：R285[医药卫生—中药学]