[Co(phen)_3]^(3+)和SWCNTs促进钌(Ⅱ)配合物对牛血清蛋白的电催化氧化  

Electrocatalytic Oxidation of Bovine Serum Albumin Mediated by Ruthenium( Ⅱ) Complexes upon Incorporation of [Co( phen)_3]^(3+) and SWCNTs

作　　者：蔡雪萍 陈琳琳[1] 许旋[1] 李红[1] 

机构地区：[1]华南师范大学化学与环境学院,广州510006

出　　处：《华南师范大学学报（自然科学版）》2015年第2期32-38,共7页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21271075);广东省自然科学基金项目(10351063101000001;S2012010008763)

摘　　要：应用循环伏安法、微分脉冲伏安法、荧光光谱法和扫描电镜等方法研究了[Co(phen)3]3+(phen=1,10-邻菲咯啉)和单壁碳纳米管(SWCNTs)促进[Ru(bpy)2(tatp)]2+(bpy=2,2'-联吡啶,tatp=1,4,8,9-四氮三联苯)在铟锡氧化物(ITO)电极上对牛血清蛋白(BSA)的电催化氧化.结果表明,[Ru(bpy)2(tatp)]2+、BSA、[Co(phen)3]3+和SWCNTs间相互作用明显,BSA-SWCNTs的加入促进了[Ru(bpy)2(tatp)]2+在ITO上的电化学组装,[Co(phen)3]3+的加入则促进了组装到ITO电极上的[Ru(bpy)2(tatp)]2+对BSA的电催化氧化,在连续微分脉冲伏安图上呈现一个类似于色氨酸电催化氧化的特征氧化峰.通过改变离子强度、Ru(Ⅱ)或Co(Ⅲ)配合物配体结构和BSA浓度的研究发现,增强BSA与双金属键合剂间作用的因素有利于Co(Ⅲ)配合物诱导Ru(Ⅱ)配合物对BSA的电催化氧化.此外,BSA的电催化氧化响应在0.3~1.5μmol/L BSA浓度区呈线性增加变化,Co(Ⅲ)配合物的伏安响应呈线性减小.研究结果有助于理解双金属键合剂对蛋白质的电催化氧化作用,为蛋白质电化学传感器的构建提供强有力的依据.[Ru（ bpy）2（ tatp） ]2 ＋（ bpy = 2,2＇-bipyridine and tatp = 1,4,8,9-tetra-aza-triphenylene）,single-walled carbon nanotubes（ SWCNTs） and [Co（phen）3]^3＋（ phen = 1,10-phenanthroline） are successfully used to promote the electrochemical oxidation of bovine serum albumin（ BSA） on an indium-tin oxide（ ITO） electrode by means of cyclic voltammetry,differential pulse voltammetry,fluorescence spectroscopy and scanning electron microscope.There are obvious interactions among [Ru（ bpy）2（ tatp） ]2 ＋,BSA,[Co（phen）3]^3＋and SWCNTs. In the present protocol,the addition of BSA-SWCNTs is found to promote the electrochemical assembly of [Ru（ bpy）2（ tatp） ]3 ＋on the ITO electrode,and the simultaneous presence of [Co（phen）3]^3＋and SWCNTs facilitates the electrocatalytic oxidation of BSA mediated by [Ru（ bpy）2（ tatp） ]2 ＋assembled on the ITO surface. BSA shows a characteristic oxidation peak,which is similar to that of tryptophan（ Trp） under our experimental conditions. The evidences from the effects of ionic strength,the structure of Ru（ Ⅱ） and Co（ Ⅲ） complexes and BSA concentration suggest that the strong binding interaction of Ru（ Ⅱ） and Co（ Ⅲ） complexes to BSA facilitates the electrocatalytic oxidation of BSA. In addition,the electrocatalytic oxidation response of BSA shows a linear increase in the range from 0. 3 to1. 5 μmol / L,and the Co（ Ⅲ）-based voltammetric current exhibits a linear decrease. The present study helps us understand the electrocatalytic oxidation of proteins mediated by double metal binding agents,as well as provides asignificant basis for protein electrochemical sensor.

关 键 词：牛血清蛋白 钌(Ⅱ)配合物 钴(Ⅲ)配合物 碳纳米管 电催化氧化 

分 类 号：O657.1[理学—分析化学]