水稻低Cd累积相关基因RNA干扰载体及根特异性表达载体的构建  

Construction of RNAi and root-specific expression vectors of genes related to low Cd accumulation in rice

作　　者：陈煜娴[1] 冯珊珊[1] 柴兴苹[1] 柴团耀[1] 

机构地区：[1]中国科学院大学生命科学学院,北京100049

出　　处：《中国科学院大学学报（中英文）》2015年第2期185-191,共7页Journal of University of Chinese Academy of Sciences

基　　金：国家自然科学基金(31370281);转基因生物新品种培育重大专项(2009ZX08009-130B)资助

摘　　要：Cd极易被水稻等作物吸收并伴随食物链在人体内累积,严重威胁人类健康.基于水稻Cd累积机制的现有研究,克隆与水稻Cd累积相关的基因Os LCD和Os HMA3,并克隆一个维管组织特异性表达的启动子Os MTP11P,利用Gateway技术及传统酶切、连接方法,成功构建适用于水稻等单子叶植物农杆菌转化的RNA干扰载体pRI-M-LCD和根特异性表达载体p CB2022-H.期望通过这2个载体共转化水稻,使大量的Cd扣留在根细胞的液泡中,限制Cd向地上部分及籽粒中的转运,从而实现Cd在水稻籽粒中的低累积.Cd accumulated in soil can be easily absorbed by rice and enriched in the human body through food chain. Investigation of the mechanism for the low Cd accumulation in rice has an important practical value for standard rice breeding and safety of food crops. Multiple genes are involved in the process of the absorption,transport,and accumulation of Cd in rice. The technique of multi-gene co-transformation facilitates the genetic control of low Cd accumulation in rice grain.We cloned two genes that are related to this process,i. e.,Os LCD and Os HMA3,and a vascularspecific promoter Os MTP11 P. The RNAi vector pRI-M-LCD and the root-specific expression vector p CB2022-H were constructed by using the gateway technology and traditional enzyme digestion and ligation methods. Through Agrobacterium tumefaciens-mediated transformation,the transgenic rice plants containing both the two transgenes are expected to sequester more Cd into the root vacuoles and to reduce the root-to-shoot translocation of Cd,resulting in low Cd accumulation in grains. The successful construction of the two vectors will be useful in molecular regulation of low Cd accumulation in rice and will lay the foundation for breeding new varieties of rice that accumulates lower Cd in grains.

关 键 词：水稻 RNA干扰 组织特异性表达 低镉累积 多基因转化 载体构建 

分 类 号：S511[农业科学—作物学]