豚鼠醛化红细胞在人流感病毒血凝活性测定中的应用  

Application of Hydroformylated Guinea Pig Red Blood Cells in Hemagglutination Test for Human Influenza Virus

作　　者：万永虎[1] 张德著[1] 郑勤妮[1] 庄丽[1] 付琳[1] 任丽娟[1] 杨桃梅 唐光鹏[1] 王昭孝[1] 

机构地区：[1]贵州省疾病预防控制中心,贵阳550004 [2]山东大学医学院,济南250012

出　　处：《中国畜牧兽医》2015年第3期569-574,共6页China Animal Husbandry & Veterinary Medicine

基　　金：贵州省疾病预防控制中心科学技术基金(2013-E2-4)

摘　　要：在流感疫情监测中,血凝试验是最常用的检测方法。针对鸡红细胞不能凝集流感病毒"O"相毒株,新鲜红细胞需现采现配、麻烦、费时,易溶血、不易保存等问题,本试验对豚鼠醛化红细胞制备方法的筛选和制备条件的优化进行研究,并以新鲜豚鼠红细胞为对照,用于流感病毒不同型别血凝试验。结果表明,戊二醛固定法制备的豚鼠醛化红细胞不溶血、结果稳定性好、保存期长、血凝活性与新鲜豚鼠红细胞基本一致,可以替代新鲜豚鼠红细胞用于血凝试验。Hemagglutination test was a conventional detection method in epidemic surveillance of influenza.In order to overcome the defects of fresh red blood cells for troublesome and time-consuming preparation,easy hemolysis,short time conservation as well as chicken red blood cells could not agglutinate influenza virus＂O＂phase strains,our research optimized the method and the condition of preparation of hydorfomylated guinea pig red blood cells through HA test for different types of influenza virus and the fresh guinea pig red blood cells were taken as control.The results showed that guinea pig red blood cells hydorfomylated by glutaraldehyde were anhemolytic,and it had a good stability and a long time conservation.Compared with fresh guinea pig red blood cells,the effect of hemagglutination activity was almost identical.

关 键 词：流感病毒 醛化红细胞 血凝试验 

分 类 号：S852.5[农业科学—基础兽医学]