麻叶荨麻醇提液对大鼠离体子宫运动的影响  

Effect of Alcohol Extract of Urtica cannabina on the Motility of Rat Uterus invitro

作　　者：周玉东[1] 赵红琼[1] 张丹[1] 王丽芳[1] 张伯伟[1] 刘璐[1] 张晓红[1] 刘朝晖[1] 

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052

出　　处：《中国畜牧兽医》2015年第3期613-618,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家青年科学基金(31201872);新疆维吾尔自治区普通高校重点学科(基础兽医学);新疆农业大学项目(sysblx016)

摘　　要：为探究荨麻对子宫运动的影响及其可能机制,本试验用生物信号处理系统测定了浓度递增(0.5%、1.0%、2.0%、4.0%、8.0%和16.0%)的麻叶荨麻(Urtica cannabina,UC)醇提液对未孕大鼠离体子宫收缩频率、收缩张力和收缩幅度的影响,并用特异性M-受体阻断剂硫酸阿托品(atropine,A,2.5μg/mL)和2.0%UC联合用药,分为3个处理:UC单独处理、UC+A和A+UC,观察UC对子宫收缩的影响是否与M-受体有关。结果显示,在UC浓度递增到4.0%时收缩频率较用药前显著增加(P<0.05);浓度递增到4.0%、8.0%时,收缩幅度较用药前极显著降低(P<0.01);但浓度递增UC对子宫收缩张力影响不显著(P>0.05)。一次性加入2.0%UC后子宫收缩张力迅速增加并接近僵直,之后逐渐降低。无论UC+A或A+UC处理都使子宫收缩频率较用药前明显加快,收缩张力极显著增加(P<0.01),但2个联合用药处理组之间差异不显著(P>0.05);UC+A和A+UC处理组收缩张力较UC单独用药组均极显著降低(P<0.01)。结果表明,UC可兴奋未孕大鼠离体子宫,A可缓解UC引起的子宫僵直或张力增加,但不能阻断UC引起的子宫收缩频率增加。提示UC对未孕大鼠离体子宫的兴奋作用和激动与M-受体有一定关系,但可能还存在其他机制。To explore the effects of Urtica cannabina（UC）on uterus motility and its possible mechanism,alcohol extracts of UC at increasing doses（0.5%,1.0%,2.0%,4.0%,8.0% and16.0%）were added to non-pregnant rat uterus in vitro.Changes of contraction frequency,contraction tension and contraction amplitude were recorded by biological signal processing system.Moreover,the combined effects of specific M-receptor antagonist atropine sulfate（A）at the dose of 2.5μg/mL and UC at a dose of 2.0% on the motility of rat uterus were tested.There were three treatments,UC alone,UC＋A and A＋UC.The results showed that the contraction frequency was significantly increased when the concentration of UC arrived 4.0%compared with that before treatment（P0.05）;When the concentrations arrived 4.0% and 8.0%,contraction amplitudes of the uterus were extremely significantly decreased compared with that before treatment（P0.01）;However,the contraction tension was not affected by the increasing concentrations of UC（P0.05）.When 2.0% UC alone treatment,uterus contraction tension increased rapidly and was close to rigidity,and then gradually decreased.Contraction frequencies after UC＋A or A＋UC treatments were extremely significantly faster than that before treatment（P0.01）,but the difference between the two combined treatments was not significant（P0.05）.Contraction ten-sion of uterus after UC＋A and A＋UC treatments were extremely significantly reduced compared with that after UC alone treatment（P0.01）.The results indicated that UC could stimulate the contraction of rat uterus partly medicated by M-receptor,whereas there maybe were some other mechanisms involved in the effect.

关 键 词：麻叶荨麻 子宫 离体 平滑肌 

分 类 号：S853.7[农业科学—临床兽医学]