卵丘细胞对猪卵母细胞体外成熟及孤雌胚胎体外发育的影响  

Effect of COCs on in vitro Maturation and Parthenogenetic Development of Pig Oocyte

作　　者：岑文[1] 华再东[2] 郑新民[2] 肖红卫[2] 张立苹[2] 吴民耀[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710119 [2]湖北省农业科学院畜牧兽医研究所,动物胚胎工程与分子育种湖北省重点实验室,武汉430064

出　　处：《中国畜牧兽医》2015年第3期638-643,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家转基因生物新品种培育重大专项(2014ZX08010-003;2014ZX08006-003);湖北省农业科技创新中心课题(2011-620-001-003);湖北省自然科学基金重点课题(2010CDA120)

摘　　要：为探讨表皮生长因子(epidermal growth factor,EGF)的添加浓度及脱卵丘细胞时间对猪卵母细胞体外成熟及孤雌胚胎体外发育的影响。试验通过在体外成熟液中添加不同浓度(0、10、15、20、30、40ng/mL)的EGF来研究其对培养44h的卵母细胞成熟率以及孤雌胚胎发育的影响;在培养开始后的不同时间(18、24、38、44h)进行脱卵丘细胞处理来研究不同时间脱卵丘处理对培养44h的卵母细胞成熟率以及孤雌胚胎发育的影响。结果表明,成熟培养基中添加10ng/mL EGF能显著提高卵母细胞的卵裂率和囊胚率(P<0.05)。共培组和独培组卵母细胞培养18h后脱卵丘细胞成熟率均低于44h,但差异不显著(P>0.05);共培组卵母细胞培养18h后脱卵丘细胞的卵裂率和囊胚率显著高于培养44h(P<0.05);独培组卵母细胞培养18h后脱卵丘细胞的卵裂率与44h无显著差异(P>0.05),但囊胚率显著高于培养44h后脱卵丘细胞(P<0.05)。添加10ng/mL EGF对猪卵母细胞体外成熟及孤雌胚胎体外发育较好;卵母细胞培养18h后脱卵丘细胞可提高孤雌胚胎早期发育能力。This experiment was conducted to study the effect of the concentration of epidermal growth factor（EGF）and times of oocytes got rid of cumulus cells on in vitro maturation and development of parthenogenetic embryoes of pig oocytes.The embryos were treated with different concentrations（0,10,15,20,30,40ng/mL）of EGF for 44 h,maturation rate,cleavage rate and blastocyst rate of oocytes were compared.Removing cumulus cell in different time（18,24,38,44h）after cultivation to study effect of the time of removing the cumulus cell on maturation rate and the developmental capacity of parthenogenetic embryos.The results showed that the cleavage rate and blastocyst rate of oocytes in maturation media with 10ng/mL EGF were significantly higher than blank treatment groups（P0.05）.There were no significant differences in the maturation rates of removing cumulus cell after cultivation of 18 hand cultivation of 44h（P0.05）;The cleavage rate and blastocyst rate of oocytes of removing cumulus cell after cultivation of 18 hwere significantly higher than cultivation of 44 hin the together culture group（P0.05）;There were no significant differences in the cleavage rate of removing cumulus cell after cultivation of18 hand cultivation of 44h（P0.05）,but the blastocyst rate of oocytes of removing cumulus cell after cultivation of 18 hwere significantly higher than cultivation of 44 hin the alone culture group（P0.05）.These results indicated that the treatment of 10ng/mL EGF was better for porcine oocyte culture in vitro and parthenogeretic development.Removing cumulus cell after cultivation of 18 hcould improve the development of parthenogenetic embryos.

关 键 词：猪 EGF 卵丘细胞 体外成熟 胚胎发育 

分 类 号：S814.6[农业科学—畜牧学]