京海黄鸡杂交组合亲本鸡球虫抗性指标的比较  

Comparison of Resistant Indexes of Coccidiosis between the Two Hybrid Parents of Jinghai Yellow Chicken

作　　者：林雨鑫[1,2] 张菁菁[1,2] 戴国俊[1,2] 张跟喜[1,2] 谢恺舟[1,2] 王金玉[1,2] 顾玉萍[3] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]江苏省动物繁育与分子标记重点实验室,扬州225009 [3]江苏京海禽业集团有限公司,海门226103

出　　处：《中国畜牧兽医》2015年第3期685-689,共5页China Animal Husbandry & Veterinary Medicine

基　　金：国家肉鸡产业技术体系项目(nyctx-42-G1-05);江苏省产学研创新资金前瞻性联合研究项目(BY2013063-06);江苏省高校自然科学研究重大项目(11KJA230001);江苏高校优势学科建设工程项目(PAPD);"十二五"国家科技支撑计划(2014BAD13B02)

摘　　要：为研究京海黄鸡杂交组合亲本BB鸡与FF鸡鸡球虫病抗性的差异,试验选取BB和FF公鸡各30只,饲养至9日龄时,每个群体随机分为免疫组(n=15)和非免疫对照组(n=15),分组当天免疫组京海黄鸡杂交组合亲本按正典鸡球虫病四价活疫苗说明书要求接种鸡球虫疫苗,疫苗免疫后第8天采用生物素双抗体酶联免疫吸附法(ELISA)测定免疫组和非免疫对照组8个血浆球虫抗性指标,并进行差异显著性检验。结果显示,BB鸡免疫组过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和白介素17(IL-17)的含量显著高于FF鸡免疫组(P<0.05);BB杂交组合亲本免疫组CAT含量极显著高于对照组(P<0.01),SOD含量显著高于对照组(P<0.05);FF杂交组合亲本免疫组CAT和谷胱甘肽过氧化物酶(GSH-Px)含量均显著高于对照组(P<0.05)。综合分析结果表明,BB鸡免疫效果优于FF鸡群体,BB鸡球虫病抗性优于FF鸡群体。In order to investigate the differences of coccidiosis resistance between Jinghai Yellow chicken hybrid parental BB and FF chickens,30 experimental cocks of BB and FF were randomly divided into vaccine group（n=15）and control group（n=15）at 9-day-old,and then the plasmas of each group were separated from each chicken at 8days post vaccination,and the resistant indexes were measured using biotin double antibody enzyme-linked immunosorbent assay（ELISA）,the significant differences between groups were tested.The results showed that the CAT,SOD,IL-17 levels in BB vaccine group were significantly higher than that in FF vaccine group（P0.05）;The CAT level in BB vaccine group was very significantly higher than that in control group（P0.01）;The SOD level in BB vaccine group was significantly higher than that in control group（P0.05）;The CAT and GSH-Px levels in FF vaccine group were significantly higher than that in control group（P0.05）.Comprehensive analysis results shows that immune effects of BB vaccine group was superior to that of FF vaccine group,and coccidiosis resistance of BB chickens was superior to that of FF chickens.

关 键 词：球虫免疫 抗性指标 杂交组合亲本 

分 类 号：S852.723[农业科学—基础兽医学]