眼镜蛇缓慢葡萄球菌的分离鉴定与药敏试验  

Isolation,Identification and Drug Susceptibility Test of Staphylococcus lentus in Cobra

作　　者：苏接瑜 廖黎黎[1] 曾芸[1,2] 李军[3,2] 

机构地区：[1]广西大学动物科学技术学院,南宁530004 [2]广西畜禽疫苗新技术重点实验室,南宁530001 [3]广西兽医研究所,南宁530001

出　　处：《中国畜牧兽医》2015年第3期721-727,共7页China Animal Husbandry & Veterinary Medicine

基　　金：广西南宁市青秀区科学技术局项目(2012N02)

摘　　要：对广西南宁市某眼镜蛇养殖场送检的患病眼镜蛇进行病理剖检及病原菌分离鉴定,根据临床发病症状、病理变化、病理组织学检查、病原分离鉴定及致病力试验结果,确诊为缓慢葡萄球菌感染。该分离菌对小鼠有一定的致病性;药敏试验结果显示,分离菌对阿莫西林钠舒巴坦钠、头孢哌酮钠舒巴坦钠、氨苄西林钠、阿莫西林钠、苯唑西林钠、哌拉西林钠、阿米卡星敏感,对头孢曲松钠、氧氟沙星、利福平中介,对链霉素、林可霉素、磺胺间甲氧嘧啶、氟苯尼考、土霉素、诺氟沙星耐药。选择敏感抗生素阿莫西林钠舒巴坦钠添加在水和料中预防发病,患病眼镜蛇肌肉注射头孢哌酮钠舒巴坦钠,46条病蛇经一个疗程治疗,仅5条死亡,效果显著,蛇群无新病例发生,1周后逐渐恢复采食。Pathogenic bacteria strain was isolated and identified from diseased cobra cultured in a cobra farm in Nanning city,Guangxi.Through a comprehensive analysis of the clinical symptoms,pathological changes,histopathological examination,pathogenic bacteria isolation and identification,and pathogenicity test,it was confirmed as Staphylococcus lentus disease.The isolate had pathogenicity to mice.The result of drug susceptibility test showed that this strain was highly sensitive to amoxicillin sodium and sulbactam sodium,cefoperazone sodium and sulbactam sodium,ampicillin sodium,amoxicillin sodium,oxacillin sodium,piperacillin sodium,amikacin;Intermediate to ceftriaxone sodium,ofloxacin,rifampin;And resistant to streptomycin,lincomycin,sulfamonomethoxine,florfenicol,terramycin,norfloxacin.Amoxicillin sodium and sulbactam sodium was added in water and fodder to prevent the disease,and cefoperazone sodium and sulbactam sodium was used to treat diseased cobra by intramuscular injection,through a course of treatment,only five of forty six diseased cobras were dead,effect was remarkable.No new cases came up in cobra herd.After one week administration,the appetites of diseased cobras were recovered gradually.

关 键 词：眼镜蛇 缓慢葡萄球菌 分离 鉴定 16SrRNA 药敏试验 

分 类 号：Q78[生物学—分子生物学]