STAT3基因siRNA表达载体的构建及鉴定  

作　　者：刘洋[1] 陈立军[2] 呼文亮[1] 

机构地区：[1]内蒙古医学院附属医院临床检验中心 [2]武警医学院生物化学教研室

出　　处：《科学咨询》2015年第3期4-6,共3页

摘　　要：目的：本研究利用RNA干扰技术，以Stat3为靶基因，设计构建重组体，并进行序列分析鉴定。方法：设计有小发夹结构的一条DNA序列，PCR扩增合成SECs表达框架，克隆至载体psiLentGeneTM中构建重组体，转化E．coli DH5α菌株，提取质粒进行酶切鉴定后测序分析。结果：酶切电泳测序分析表明，插入序列正确，重组质粒构建成功。结论：STAT3靶向RNA干扰重组体的成功构建和序列分析。Objective： To clone the recombinant plasmid affecting gene STAT3 translation by RNA interfering and analyze the nucleic acid sequence of the recombinant. Methods： The design has a small hairpin structure of DNA sequence, PCR amplification SECS synthetic siRNA expression,cloning vector to construct recombinants in psiLentGeneTM into E.coli DH5α strain, extract plasmid after enzyme digestion sequencing. Results：Sequencing analysis showed that the enzyme electrophoresis, insertion sequence is correct, the recombinant plasmid was successfully constructed. Conclusion： STAT3 targeting recombinant RNA interference was successfully constructed and sequence was analysised.

关 键 词：STAT3 RNA干扰 质粒 

分 类 号：Q756[生物学—分子生物学]