孕酮对反复自然流产小鼠白介素8表达的调节作用  

Effect of progesterone on expression of interleukin 8 in mice with repeated spontaneous abortion

作　　者：宋芳[1] 冯若鹏[1] 赵紫薇[1] 陈晶[1] 贺帅[2] 陈小艳[2] 

机构地区：[1]包头医学院组织学与胚胎学教研室,014010 [2]包头医学院形态学实验室

出　　处：《中国计划生育学杂志》2015年第3期161-164,168,共5页Chinese Journal of Family Planning

基　　金：内蒙古自治区高等学校科学技术研究(NJ10192)

摘　　要：目的:探讨孕酮对自然流产小鼠母胎界面白介素(IL)-8的调节作用。方法:建立经典的自然流产小鼠模型(CBA/J×DBA/2交配组合),分为两组,孕酮组注射黄体酮,模型组同步注射等量的生理盐水。另设正常妊娠组小鼠(正常组)对照,每组10只小鼠。用免疫组织化学染色法和反转录-聚合酶链式反应(RT-PCR)技术检测各组小鼠蜕膜及绒毛组织中IL-8蛋白和mRNA的相对表达情况。结果:孕酮组血清孕酮(84.91±10.05ng/ml)水平明显高于模型组(41.83±9.77ng/ml),而IL-8(615.23±213.62pg/ml)水平明显低于模型组(1653.14±321.25pg/ml),差异均有统计学意义(P<0.01);孕酮组血清孕酮、IL-8水平分别与正常组比较,差异均无统计学意义(P>0.05)。免疫组织化学染色和RT-PCR在绒毛组织中,孕酮组IL-8蛋白和mRNA(0.067±0.028,61.28±4.13)相对含量均明显低于模型组(0.135±0.012,85.72±2.63),差异均有统计学意义(P<0.01),与正常组(0.059±0.021,58.33±2.87)比较均无统计学意义(P>0.05);在蜕膜组织中,孕酮组IL-8蛋白和mRNA的相对含量低于模型组,差异均有统计学意义(P<0.05),与正常组比较均无统计学意义(P>0.05)。结论:孕酮可下调自然流产小鼠母胎界面IL-8的表达。Objective：To explore the role of progesterone on the expression of interleukin（IL）8on fetal maternal interface in mice with repeated spontaneous abortion.Methods：A classical（CBA/J×DBA/2）animal model of spontaneous abortion was set up,and the expression of IL-8protein and mRNA of decidua and villi in spontaneous abortion mice was measured by immunohistochemical staining method and reverse transcription-polymerase chain reaction（RT？PCR）.Results：In the progesterone group,the serum concentration of progesterone[（84.91±10.05）ng/ml]was significantly higher than that in the model group[（41.83±9.77）ng/ml],while the IL-8level[（615.23±213.62）pg/ml]was significantly lower than that in the model group[（1653.14±321.25）pg/ml,P〈0.01].There were no significant differences in serum IL-8and progesterone concentrations between the progesterone group and the control group（Pall〉0.05）.The results of immunohistochemical staining and RT-PCR showed that in the chorionic villi,relative contents of IL-8protein and mRNA（0.067±0.028,61.28±4.13）in the progesterone group were lower than those in the model group（0.135±0.012,85.72±2.63）,showing significent difference（P〈0.01）;there were no significant difference in relative contents of IL-8protein and mRNA in the chorionic villi between progesterone group and the normal group（0.059±0.021,58.33±2.87,P〉0.05）.In addition,in the decidual tissue,the relative contents of IL-8protein and mRNA in the progesterone group were significantly lower than those in the model group（P〈0.05）,while similar with those of the normal group（P〉0.05）.Conclusion：Progesterone could down-regulate the expression of IL-8on fetal maternal interface in spontaneous abortion mice.

关 键 词：白介素8 自然流产 孕酮 免疫组织化学 多聚酶链反应 动物模型 

分 类 号：R714.21[医药卫生—妇产科学]