两面针商品药材的质量评价  

Quality Evaluation of Medicinal Material of Zanthoxyli Radix

作　　者：张振山[1] 严萍[1] 谭志滨 林文华[1] 童家赟[2] 詹若挺[1] 

机构地区：[1]广州中医药大学中药资源科学与工程研究中心、岭南中药资源教育部重点实验室,广州510006 [2]广州中医药大学中药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第6期57-61,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中央财政支持地方高校发展专项资金项目-中药学(粤财教[2013]389号);广东省科技计划项目(2012A030100006)

摘　　要：目的：对市售两面针商品药材进行质量调查和评价。方法：参照2010年版《中国药典》两面针项下方法,对18批两面针商品药材进行性状鉴别、薄层色谱及高效液相法检测氯化两面针碱含量,并建立两面针HPLC指纹图谱方法,结合相似度评价对样品药材进行鉴别分析。结果：样品性状,薄层鉴别和含量测定的结果显示,9批药材有伪品掺杂现象;样品总灰分的含量是2.4%-6.4%,水分含量是7.0%-12.1%,醇溶性浸出物含量是2.2%-8.6%;HPLC测定中有2批样品检测不到氯化两面针碱,11批样品图谱与对照指纹图谱相似度低于0.8;18批药材均不符合2010年版《中国药典》标准。结论：目前市场上的两面针药材品质参差不齐,伪品掺杂现象比较严重。Objective： To evaluate the quality of medicinal material of Zanthoxyli Radix in Chinese herbal medicine market.Method： The 18 batches of commercial medicinal material of Zanthoxyli Radix were identified and analysed by macroscopical identification,TLC,HPLC according to the Chinese Pharmacopoeia published in 2010.HPLC fingerprint was applied to evaluate the quality of Zanthoxyli Radix.Result： There were adulterants detected in 9 batches by the macroscopical identification,TLC and HPLC.The total ashes were between 2.4% and 6.4%,the moisture ranged from 7.0% to 12.1%,and the alcohol-soluble extract was in the range of 2.2% to 8.6%.Nitidine chloride was not be detected in 2 batches by HPLC.Compared to the HPLC fingerprint common mode of Zanthoxyli Radix,the similarity of 11 batches were less than 0.8.The 18 batches of samples were not complied with Chinese Pharmacopoeia in 2010.Conclusion： Currently,the quality of medicinal material of Zanthoxyli Radix in Chinese herbal medicine market varies greatly,and the phenomenon of adulterants doping is serious.

关 键 词：两面针 商品药材 质量评价 薄层色谱法 高效液相色谱法 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]