HPLC同时测定白花丹参中丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ和二氢丹参酮Ⅰ的含量  

Simultaneous Determination of Tanshinone ⅡA,Tanshinone Ⅰ,Cryptotanshinone and Dihydrotanshinone Ⅰ in Salviae Albae Radix by HPLC

作　　者：张琳琳[1] 王淳[2] 宋志前[2] 杜智勇[2] 宁张弛 董运茁 刘振丽[2] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310003 [2]中国中医科学院中医基础理论研究所,北京100700 [3]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2015年第6期62-65,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立同时测定白花丹参中丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量的方法。方法：从白花丹参产地山东采集了10个批次白花丹参,采用HPLC测定4种主要丹参酮成分丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量。色谱条件：Zorbax Extend-C18色谱柱（4.6mm×150mm,5μm）,流动相乙腈-0.2%乙酸水（57∶43）,流速1.0mL·min-1,检测波长270nm,柱温30℃。结果：丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ分别在0.138-2.76（r=0.999 9）,0.024 8-0.496（r=0.999 9）,0.096-1.92（r=0.999 9）和0.035-0.70μg（r=0.999 9）线性关系良好。加样回收率分别为100.06%,99.60%,99.91%,100.06%。丹参酮ⅡA、丹参酮Ⅰ、隐丹参酮和二氢丹参酮Ⅰ含量范围分别是2.677-7.150,0.320-1.747,0.916-5.935,0.132-2.601 mg·g-1。结论：该法简便、准确、分离度好,能同时测定白花丹参中4种有效成分的含量。Objective： To establish the method for simultaneous determination of tanshinone ⅡA,tanshinone I,cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix by HPLC.Method： Each of ten batches of Salviae Albae Radix were collected from the same place-Shandong province.A simple and effective highperformance liquid chromatographic（HPLC） method has been developed for simultaneous quantification of four main diterpenes（tanshinone IIA,tanshinone I,cryptotanshinone,dihydrotanshinone I） in Salviae Albae Radix.Chromatography was performed on a（4.6mm × 150mm,5μm） particle size,extend-C18 column.The mobile phase was a linear gradient prepared from 0.2% aqueous formic acid and acetonitrile（43∶ 57） at a flow-rate of 1.0mL·min-1.Detection was accomplished at 270nm and column temperature was set at 30 ℃.Result： The contents of tanshinone IIA,tanshinone I,cryptotanshinone,dihydrotanshinone I in Salviae Albae Radix were between 2.677-7.150,0.320-1.747,0.916-5.935,0.132-2.601mg·g-1,repectively.The average recovery was 100.06%,99.60%,99.91%,100.06%,repectively.Conclusion： The developed method is simple,accurate and good separation, which is suitable for the quality control of tanshinone ⅡA, tanshinone I,cryptotanshinone and dihydrotanshinone I in Salviae Albae Radix.

关 键 词：白花丹参 高效液相色谱 丹参酮ⅡA 丹参酮Ⅰ 隐丹参酮 二氢丹参酮Ⅰ 

分 类 号：R284.1[医药卫生—中药学]