HPLC同时测定脉平片中白果内酯、银杏内酯A和银杏内酯B、银杏内酯C的含量  

Determination of Bilobalide,Ginkgolide A,Ginkgolicle B and Ginkgolicle C in Maiping Tablet by HPLC

作　　者：倪琳 张秀娟[2] 高进贤[3] 杨锡 

机构地区：[1]甘肃省药品检验研究院,兰州730070 [2]兰州大学药学院,兰州730000 [3]甘肃省人民医院,兰州730000

出　　处：《中国实验方剂学杂志》2015年第6期66-68,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立脉平片中白果内酯、银杏内酯A、银杏内酯B和银杏内酯C的含量测定方法。方法：采用HPLC对制剂中银杏叶提取物的主要成分白果内酯、银杏内酯A、银杏内酯B和银杏内酯C进行定量分析。CAPCELL PAK C18色谱柱（4.6mm×250mm 5μm）;流动相四氢呋喃-乙腈-水（12∶10∶78）,蒸发光散射检测器,柱温30℃,流速1.0mL·min-1。结果：银杏内酯C在1.11-6.66μg,白果内酯在2.232-13.392μg,银杏内酯A在1.912-11.472μg,银杏内酯B在1.502-9.004μg均呈良好的线性关系,平均回收率分别为96.36%,97.18%,97.71%,96.57%;RSD分别为1.0%,1.4%,2.1%,1.4%。结论：方法准确可靠,专属性强,结果稳定,重复性好,可有效控制制剂质量。Objective： To establish an method for determination bilobalide,ginkgolide A,ginkgolicle B and ginkgolicle C in Maiping tablet.Method： The bilobalide,ginkgolide A,ginkgolicle B and ginkgolicle C in Maiping tablet was determined by HPLC.CAPCELL PAK C18（4.6mm × 250mm 5μm） column was used.The modbile phase was tetrahydrofuran-acetonitrile-water（12 ∶ 10 ∶ 78） with the flow rate of 1.0mL·min-1.The column temperature was 30 ℃ and evaporative light-scatting mass detector.Result： The ginkgolicle C,bilobalide,ginkgolide A and ginkgolicle B were linear at the range of 1.11-6.66,2.232-13.392,1.912-11.472,1.502-9.004μg,respectively.The average recovery was 96.36%,97.18%,97.71%,96.57% and RSD was1.0%,1.4%,2.1%, 1.4%（n = 6）.Conclusion： The established method is accurate, reliable and specific.The results are stable with good reproducibility.The quality of Maiping tablet can be controlled by the method.

关 键 词：白果内酯 银杏内酯A 银杏内酯B 银杏内酯C 高效液相色谱 脉平片 

分 类 号：R284.1[医药卫生—中药学]