江苏省沿海滩涂中华大蟾蜍种群的遗传多样性分析  

Genetic Diversity Analysis of Bufo gargarizans Population in the Coastal Wetland of Jiangsu Province

作　　者：王兰萍[1] 耿荣庆[2] 朱华杰[2] 郑慧[2] 王梦迪[2] 张子锁 

机构地区：[1]盐城师范学院生命科学与技术学院,江苏盐城224051 [2]盐城师范学院药学院,江苏盐城224051

出　　处：《安徽农业科学》2015年第8期93-94,共2页Journal of Anhui Agricultural Sciences

基　　金：江苏省高等学校大学生创新创业训练计划项目(201310324050X)

摘　　要：[目的]为中华大蟾蜍野生种群资源的保护和合理开发利用提供理论依据。[方法]通过对中华大蟾蜍线粒体控制区的部分序列进行测序分析,研究江苏沿海地区中华大蟾蜍不同地域种群的遗传多样性。[结果]在江苏省沿海滩涂的3个地域检测到中华大蟾蜍种群的6个单倍型类型,东台种群、大丰种群和射阳种群的个体间享有共享的单倍型类型,其余单倍型均是各种群独享。系统发育树分析表明,中华大蟾蜍3个地域种群没有形成明显的地理分布格局。[结论]各地域种群间既存在着一定程度的基因交流,也存在一定程度的遗传分化。[ Objective ] The research aimed to provide theoretical basis for the protection and reasonable development and utilization of wild re- sources of Bufo gargarizans populatian. [ Method ] Based on the sequencing analysis of partial sequence of the mitochondrial control region of B. gargarizans, the genetic diversity of different geographical population of B. gargarizans in the coastal wetland of Jiangsu Province was studied. [ Result ] Six haplotypes were detected from three geographical populations in the coastal wetland of Jiangsu Province. The individuals in Dongtai population, Dafeng population and Sheyang population shared the same haplotypes. But the other haplotypes were exclusive. The results of phylo- genesis analysis showed that no obvious geographic distribution pattern was found among three geographical populations of B. gargarizans. [ Con- clusion] A certain degree of gene flow and ~enetic differentiation existed among different geographical populations.

关 键 词：中华大蟾蜍 线粒体控制区 遗传多样性 系统发育树 

分 类 号：S917[农业科学—水产科学]