小菜蛾抗菌肽聚乳酸-羟基乙酸共聚物纳米粒的制备及体外评价  

Preparation and in vitro evaluation of antibacterial peptides from Plutella xylostella poly(lactic-co-glycolic acid) nanoparticles

作　　者：刘朝勇[1,2] 肖云芝[1,2] 李瑞生[2] 李晓荣[1,2] 韩晋[2] 袁海龙[2] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]中国人民解放军第三○二医院,北京100039

出　　处：《中草药》2015年第3期348-352,共5页Chinese Traditional and Herbal Drugs

基　　金：国家新药创制重大专项(2011ZX092J12108-04C)

摘　　要：目的制备小菜蛾抗菌肽（CA）聚乳酸-羟基乙酸共聚物（PLGA）纳米粒（CA-PLGA-NPs）,并对其理化性质及体外释放度进行研究。方法采用S/W/O/W复乳法结合高压均质法制备CA-PLGA-NPs,对其纳米粒的形态、粒径、多分散指数（PDI）、载药量、包封率和体外释放度进行研究。结果 CA-PLGA-NPs呈球形或类球形,粒径、PDI、载药量、包封率分别为（358.76±22.51）nm、0.168 1±0.012 2、（10.50±0.28）%、（60.92±1.58）%,突释现象不明显,2~10 d内达到释药稳定期。结论 S/W/O/W复乳法结合高压均质法制备CA-PLGA-NPs工艺可行,为小菜蛾抗菌肽给药提供了制剂学基础。Objective To prepare the antibacterial peptides from Plutella xylostella-loaded nanoparticles based on poly（lactic-co- glycolic acid） （CA-PLGA-NPs） and evaluate its physicochemical property and in vitro release. Methods CA-PLGA-NPs were prepared by S/W/O/W double emulsion method combined with high-pressure homogenization. The morphology, particle size, polydispersion index （PDI）, drug loading, encapsulation efficiency （EE）, and in vitro release of the nanoparticles were studied. Results CA-PLGA-NPs were spherical or similarly spherical, and the average particle size, PDI, drug loading, and EE were （358.76 ±22.51） nm, 0.168 1 ± 0.012 2, （10.50 ± 0.28）%, and （60.92 ±1.58）%, respectively. And burst phenomenon was not significant. The drug delivery stable phase was 2-10 d. Conclusion S/W/O/W double emulsion method combined with high pressure homogenization method is suitable for the preparation of CA-PLGA-NPs, and provides a pharmaceutical basis for CA administration.

关 键 词：小菜蛾抗菌肽 S/W/O/W复乳 高压均质 聚乳酸-羟基乙酸共聚物 载药量 包封率 

分 类 号：R283.6[医药卫生—中药学]