异绿原酸A、B和C的制备工艺研究  

Preparation technology of isochlorogenic acids A, B, and C

作　　者：倪付勇[1,2] 宋亚玲[1,2] 刘露[1,2] 赵祎武[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222000 [2]中药制药过程新技术国家重点实验室,江苏连云港222000

出　　处：《中草药》2015年第3期369-373,共5页Chinese Traditional and Herbal Drugs

基　　金：科技部重大新药创制:现代中药创新集群与数字制药技术平台(2013ZX09402203)

摘　　要：目的建立从金银花Lonicera japonica中分离制备高质量分数异绿原酸A、B和C的方法。方法采用D-101大孔树脂、中低压制备色谱分离制备金银花中异绿原酸A、B和C单体,根据理化性质和波谱数据鉴定其结构。结果分离制备的异绿原酸A、B和C质量分数分别为98.7%、99.2%和97.6%。结论该方法经济、简单、快速,可用于制备高质量分数的异绿原酸A、B和C。Objective To establish a method for separation of isochlorogenic acids A, B, and C from Lonicerae Flos. Methods Isochlorogenic acids A, B, and C in Lonicerae Flos were isolated and purified by macroporous resin and medium-low-pressure preparative chromatography. Their structures were identified on the basis of the spectral data and physicochemical property. Results The contents of prepared isochlorogenic acids A, B and C were 98.7%, 99.2%, and 97.6%, respectively. Conclusion This method is economic, simple, rapid, and effective for the preparation of isochlorogenic acids A, B, and C with high purity.

关 键 词：异绿原酸A 异绿原酸B 异绿原酸C 金银花 D-101大孔树脂 中低压制备色谱 

分 类 号：R284.2[医药卫生—中药学]