Molecular Phylogeny of Pamphagidae(Acridoidea,Orthoptera) from China Based on Mitochondrial Cytochrome Oxidase I Sequences  

基于cox1基因全序列的癞蝗科部分类群系统发育关系分析（英文）

作　　者：宋卫卫[1] 郝金凤[1] 赵晓雅 温翠娇[1] 张道川[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《Agricultural Science & Technology》2015年第2期228-234,共7页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31272342);the 2ndGroup Innovative Foundation Project for Students in China in 2012(201210075004)~~

摘　　要：Phylogenetic relationships of Pamphagidae were examined using cytochrome oxidase subunit I（cox1） gene sequences.Eleven species of Pamphagidae from 7 genera were sequenced to obtain mt DNA data,along with 2 species from the Gen Bank nucleotide database.The results of sequence comparisons showed the cox1 gene is 1 534 bp in length,including 326 varible sites and 211 parsimonious information sites.The percentage of A ＋T is 67.1% in the nucleotide sequences,showing a strong AT bias.Genetic distances among subfamilies are 0.08.Using Locusta migratoria as outgroup,the phylogenetic trees were reconstructed with NJ,MP,ML and Bayesian inferences,and the results showed that the clustering results were approximately identical to that of classical morphological classification.Thrinchinae and Pamphaginae were a monophyletic group,respectively.Two species of the genus Asiotmethis of Prionotropisinae did not get together with other species of Prionotropisinae,classification position of Asiotmethis should be further discussed by both genetic markers and morphological features.The current genus Filchnerella of Prionotropisinae was not a monophyletic group.该研究测定了我国癞蝗科7属11种昆虫细胞色素氧化酶I(cox1)基因全序列,并联系从Gen Bank下载的贺兰短鼻蝗、红缘疙蝗的cox1基因全序列进行序列分析,结果表明,除宽纹蠢蝗外cox1基因全序列均为1 534bp,其中有326个变异位点,211个简约信息位点;A+T平均含量为67.1%,碱基组成具有AT偏斜;遗传距离分析发现,遗传距离0.08可以将癞蝗科很好的分为4个组。以飞蝗为外群,用NJ、MP、ML和贝叶斯法重建了癞蝗科的系统发育树,结果显示,锯癞蝗亚科的短鼻蝗属、突鼻蝗属、疙蝗属和贝蝗属4属8种蝗虫聚为一支,但短鼻蝗属的单系性未得到支持,癞蝗亚科的2种笨蝗聚为一支,蠢蝗亚科的宽纹蠢蝗为一支,与形态分类学将我国癞蝗科分为3个亚科的结果一致。锯癞蝗亚科的2种波腿蝗组成一支,没有和锯癞蝗亚科的其他种类聚在一起,波腿蝗属的分类地位有待进一步研究。

关 键 词：PAMPHAGIDAE PHYLOGENY Mitochondrial DNA Cox1 gene 

分 类 号：Q963[生物学—昆虫学]