乙肝/梅毒联合检测试纸条在献血前初筛的应用初探  

Application of the Hepatitis B / Syphilis combo strip for blood screening before donation

作　　者：古醒辉[1] 熊文[1] 叶贤林[1] 曾劲峰[1] 曾雪珍[1] 刘衡[1] 邬林枫[1] 杜丹丹[1] 

机构地区：[1]深圳市血液中心,广东深圳518035

出　　处：《中国输血杂志》2015年第2期193-196,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨献血前应用HBsAg/梅毒联合检测试纸条进行血液筛查的优势,尽量减少不合格血液的采集,避免资源浪费。方法收集经酶免方法检测的乙肝表面抗原(HBsAg)阳性或梅毒抗体阳性的血浆标本共473例,再用HBsAg/梅毒联合检测试纸条进行检测。结果用倍比稀释的方法测得HBsAg/梅毒联合检测试纸条HBsAg项目的最低检出限为2IU/mL,梅毒抗体项目的最低检出限为0.25IU/mL;经HBsAg/梅毒联合检测试纸条检测:HBsAg试纸条初筛阴性、HBsAg酶免检测阳性的标本270例,检出HBsAg阳性80例,检出率为29.63%(80/270);梅毒抗体酶免检测阳性的标本203例,检出梅毒抗体阳性140例,检出率为60.87%(140/203),与梅毒螺旋体明胶颗粒凝集实验(TPPA确证实验)的阳性检出率完全符合,符合率达100%。结论使用HBsAg/梅毒联合检测试纸条进行献血前初筛,可以筛出梅毒抗体阳性的献血者,还可以提高对HBsAg阳性献血者的筛选,既降低了阳性血液的采集,减少了资源浪费,又保证了血液安全。Objective To investigate the application of the Hepatitis B/ Syphilis combo strip for blood screening before donation,to reduce the collection of unqualified blood and to avoid waste.Methods 473 HBs Ag and TP positive plasma samples were collected,which were then confirmed by ELISA assay and were lastly tested by Hepatitis B/ Syphilis combo strips.Results The standards of HBs Ag（4 IU/mL）/ Syphilis（2 IU/mL） were gradually diluted and tested by the strips.The detection limits of HBs Ag and Anti-TP were 2 IU/ ml L and 0.25 IU/mL,respectively.Among the inconsistent samples,80 were HBs Ag strip negative/ HBs Ag ELISA positive,with a positive rate of HBs Ag at 29.63%（80/270）; 140 were antiTP strip negative/ TP ELISA positive,with a positive rate of TP at 60.87%（140/203）.The data from this method fully complied to and were consistent with the confirmatory results from TPPA.Conclusion The strip can exclude Hepatitis B/Syphilis infected blood donors from blood donation,thus reducing the numbers of unqualified blood collection and ensuring blood safety.

关 键 词：联合试纸条 血液快速检测 胶体金法 酶联免疫试验 

分 类 号：R446[医药卫生—诊断学] R512.6[医药卫生—临床医学]