利用质量控制数据评估血站ALT检测测量不确定度方法的探讨  

Investigation on the evaluation of the measurement of uncertainty in ALT test in blood center laboratory by the data of quality control

作　　者：何成涛[1] 马贵明[1] 傅强[1] 张立波[1] 

机构地区：[1]南京红十字血液中心,江苏南京210003

出　　处：《中国输血杂志》2015年第2期196-197,共2页Chinese Journal of Blood Transfusion

摘　　要：目的利用质量控制数据评估血站ALT检测测量不确定度,探讨血站实验室测量不确定度的评估方法。方法利用批内变异、批间变异、室间质评结果及校准品不确定度计算实验室ALT检测的合成不确定度及扩展不确定度。结果构成不确定的分量中,批间变异所占比例最大,其次是校准品分量和系统偏倚,最后是批内变异分量。与CLIA可接受范围相比,ALT检测结果符合要求。结论该测量不确定度评估方法简便易行,可用于血站实验室。Objective To investigate the evaluation method of the measurement of uncertainty in blood center laboratory by analyzing the data from ALT quality control.Methods The inter- and intra-assay coefficient of variability,the results from the external quality assessment and the uncertainty of the standards were used to calculate the combined and expanded uncertainties of the ALT tests in blood center laboratory.Results Among the components constituting the uncertainty,the variability of intra-assay coefficients contributed most to the measurement of uncertainty,followed by the calibrator component and systematic bias.The variability of inter-assay coefficient contributed least to the measurement of uncertainty.The results of ALT test were proved to be up to standard compared with the acceptable performance of Clinical Laboratory Improvement Amendment.Conclusion This evaluation method of the measurement of uncertainty could be easily operated in blood center laboratory.

关 键 词：测量不确定度 室间质评 质量控制 

分 类 号：R446[医药卫生—诊断学]