天然产物增强阿霉素抗乳腺癌作用的体外筛选  

Screening of natural products enhancing doxorubicin′s anti-breast cancer effect in vitro

作　　者：刘雯清[1] 张璐璐[2] 刘毅清[3] 顾文文[4] 颜天华[4] 姜远英[2] 曹永兵[1,2] 

机构地区：[1]福建中医药大学药学院,福州350108 [2]第二军医大学药学院,上海200433 [3]第二军医大学训练部,上海200433 [4]中国药科大学药学院,南京210009

出　　处：《西北药学杂志》2015年第2期152-155,共4页Northwest Pharmaceutical Journal

基　　金：上海市自然科学基金面上项目(编号:12ZR1437700)

摘　　要：目的从植物天然产物中筛选具有增强阿霉素抗乳腺癌细胞增殖作用的活性化合物,并对两药联合后的体外抗肿瘤活性进行初步评价。方法采用MTT法研究15种单体化合物对人乳腺癌细胞MDA-MB-231的影响。结果小檗碱、松萝酸、鱼腥草素钠、槲皮素在50μg·mL-1时对MDA-MB-231细胞的抑制率分别为72%(P<0.001),74%(P<0.001),91%(P<0.001)和59%(P<0.01),且具有剂量依赖性。联合用药结果显示,小檗碱与阿霉素联用,CI值小于1。结论小檗碱、松萝酸、鱼腥草素钠、槲皮素对MDA-MB-231细胞的增殖活性具有抑制作用,同时,小檗碱与阿霉素联用具有协同抗肿瘤作用。Objective To screen the active anti-tumor compounds from natural herb products and make a preliminary assessment on the anti-tumor activities of their combination with doxorubicin in vitro.Methods MTT method was used to test 15 monomeric compounds′anti-tumor effects on breast cancer cells MDA-MB-231 in vitro.Results The inhibition rates of berberine,usnic acid,sodium houttuyfonate and quercetin on MDA-MB-231 cells were 72%（P〈0.001）,74%（P〈0.001）,91%（P〈0.001）and59%（P〈0.01）at 50μg·mL^-1 in a dose-dependent manner.Drug combination results showed that CI values of berberine combined with doxorubicin was less than 1.Conclusion Berberine,usnic acid,sodium houttuyfonate and quercetin inhibited the proliferation of MDA-MB-231 cells.Drug combination results showed that berberine combined with doxorubicin had synergistic antibreast cancer efficacy.

关 键 词：天然产物 阿霉素 抗乳腺癌 联合用药 药物筛选 MDA-MB-231 小檗碱 

分 类 号：R965[医药卫生—药理学]