17β-雌二醇对小鼠肺成纤维细胞凋亡及Ⅰ型胶原表达的影响  

Effects of 17β-estradiol on apoptosis and typeⅠcollagen expression in mouse lung fibroblast

作　　者：肖永红[1] 梁晓飞[1] 王悦[1] 肖倩[1] 

机构地区：[1]河北联合大学公共卫生学院,河北唐山063000

出　　处：《工业卫生与职业病》2015年第2期98-100,106,共4页Industrial Health and Occupational Diseases

基　　金：河北省科学技术研究与发展计划(10276113D);河北省教育厅课题(2006139);唐山市科技局科技计划项目(13130269z)

摘　　要：目的探讨不同浓度17β-雌二醇(17β-E2)对小鼠肺成纤维细胞凋亡及Ⅰ型胶原表达的影响。方法将体外培养的小鼠肺成纤维细胞分为5组:空白对照组,SiO2组,SiO2+不同剂量17β-E2组(10-8、10-7、10-6 mol/L),于作用24、48、72h后收集细胞,应用流式细胞仪检测细胞凋亡,免疫组化法检测Ⅰ型胶原表达。结果与空白对照组比较,SiO2处理组小鼠肺成纤维细胞凋亡率明显降低(P<0.05),而SiO2+17β-E2处理组各时点随着17β-E2剂量增大凋亡率明显升高(P<0.05),随处理时间延长,各剂量17β-E2组成纤维细胞凋亡率明显升高(P<0.05);各处理组Ⅰ型胶原表达有明显差异,与空白对照组比较,SiO2组Ⅰ型胶原表达均明显增高(P<0.05),而17β-E2处理组Ⅰ型胶原表达均明显低于SiO2处理组,差异均有统计学意义(P<0.05)。结论 17β-E2促进小鼠肺成纤维细胞凋亡,抑制Ⅰ型胶原表达,进而抑制肺纤维化。Objective To observe the effects of different concentration of 17β-estradiol（17β-E2）on apoptosis and expression of type Ⅰ collagen in mouse lung fibroblast（LF） stimulated by SiO2.Methods Fibroblast cells were divided into five groups：blank control group,SiO2（100mg/L）group and SiO2（100mg/L）＋ different concentration of 17β-E2（10-8、10-7、10-6 mol/L）.The cells were collected after24,48 and 72h.Cell apoptosis was detected by the flow cytometry,and the expression of type Ⅰ collagen were examined with immunohistochemistry method.Results Compared with the blank control group,the apoptosis rate of the mouse lung fibroblasts treated by SiO2 was significantly decreased（P 0.05）,while which in the SiO2＋17β-E2 group was significantly increased as 17β-E2 dose increased at different time（P0.05）,and the different dose of 17β-E2 significantly stimulated the fibroblast apoptosis with the prolonging of the treatment time（P0.05）.There were significant difference in the expression of type Ⅰ collagen among the different groups（P 0.05）,which in SiO2 group were evidently higher than those in the blank control group,while which in 17β-E2 group decreased significantly compared with SiO2group（P0.05）.Conclusions17β-E2 can promote the apoptosis of mouse lung fibroblast cell and inhibit the expression of typeⅠ collagen to inhibit the lung fibrosis.

关 键 词：17Β-雌二醇 肺成纤维细胞 凋亡 Ⅰ型胶原 

分 类 号：R135.2[医药卫生—劳动卫生]