长效硝酸酯对心力衰竭大鼠肾脏血管紧张素Ⅱ受体表达水平的影响  

Effect of long-acting nitrate on expression of AngⅡreceptor subtypes in kidneys of heart failure rats

作　　者：彭余波 李艳芳[1,2] 陈萌萌[1,2] 

机构地区：[1]首都医科大学附属北京安贞医院急诊35病房 [2]北京市心肺血管疾病研究所,100029 [3]航空总医院

出　　处：《中华老年心脑血管病杂志》2015年第3期295-298,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：中国医师协会探索心血管研究基金(DFCMDA201203)

摘　　要：目的探讨长效硝酸酯异乐定对心肌梗死后心力衰竭大鼠心功能和肾脏血管紧张素Ⅱ受体(ATR)各亚型表达水平的影响。方法选用雄性10周龄Wistar大鼠90只,随机分为正常对照组9只(A组)、假手术组8只(B组),余73只结扎左冠状动脉前降支制作心肌梗死后心力衰竭模型,模型成功44只,随机分为心力衰竭模型组9只(C组)、硝酸酯小剂量组9只(D组)、硝酸酯大剂量组9只(E组)、阳性药物对照组9只(奥美沙坦酯,F组)及硝酸酯大剂量联合阳性药物组8只(G组)。灌胃法给药6周。超声心动图检测用药前后大鼠心功能,半定量RT-PCR和Western blot检测各组大鼠肾脏皮质AT1R及AT2R的表达水平。结果用药后,与B组比较,C组大鼠血浆肾素活性(PRA)、血管紧张素Ⅱ(AngⅡ)水平显著升高(P<0.01),AT2R表达明显下调(P<0.05),AT1R表达明显上调(P<0.05);与C组比较,E组、F组、G组PRA、AngⅡ明显降低(P<0.05,P<0.01),AT2R表达明显上调,AT1R表达明显下调(P<0.05);与F组比较,G组AT1R明显下调,AT2R明显上调(P<0.05)。结论长期应用长效硝酸酯在改善心功能的同时,对保护肾脏功能起到了有益的治疗作用。Objective To study the effect of long‐acting nitrate on cardiac function and expression of AngⅡreceptor （ATR）subtypes in kidneys of chronic heart failure （CHF）rats after myocardial infarction .Methods Ninety male Wistar rats aged 10 weeks were randomly divided into control group （group A ,n=9） ,sham operation group （group B ,n=8） ,HF model group （group C ,n=9） , low Elantan dose group （group D ,n=9） ,high Elantan dose group （group E ,n=9） ,olmesartan group （group F ,n=9） ,and combined high Elantan dose and olmesartan group （group G ,n=8） .A HF model was established by ligating the left anterior descending artery .The animals received gastric drugs for 6 weeks .Their cardiac function was assyed by ultrasound echocardiography and expressions of AT1 R and AT2 R were detected by RT‐PCR and Western blot ,respectively .Results The PRA and AngⅡexpression levels were significantly higher ,the AT1 R expression level was significantly higher and the AT2 R expression level was significantly lower in group C than in group B （P＆lt;0 .01 ,P＆lt;0 .05） .The PRA and AngⅡexpression levels were significantly lower ,the AT1 R expression level was significantly lower and the AT2 R expression level was significantly higher in groups E‐G than in group C （P＆lt;0 .05 ,P＆lt;0 .01） .The receptor expression levels were much higher in group G than in group F （P＆lt;0 .05） .Conclusion Long‐term use of long‐acting nitrate can effectively improve cardiac function and protect renal function .

关 键 词：受体 血管紧张素 2型 心肌梗死 心力衰竭 心血管药物 

分 类 号：R541.6[医药卫生—心血管疾病]