和厚朴酚抗小鼠急性哮喘气道炎症作用及可能机制探讨  

Effects of Honokiol on Inhibition of Airway Inflammation During Acute Asthma and its Possible Mechanism

作　　者：姚慧生[1] 韩晓华[1] 

机构地区：[1]中国医科大学附属盛京医院,辽宁沈阳110004

出　　处：《医学临床研究》2015年第1期5-9,共5页Journal of Clinical Research

摘　　要：【目的】探讨和厚朴酚对急性哮喘气道炎症的抑制作用，并分析其可能的机制。【方法】通过卵蛋白（VOA）刺激诱导小鼠急性哮喘模型，使用和厚朴酚对哮喘小鼠治疗后统计肺泡灌洗液中的中性粒细胞、淋巴细胞、巨噬细胞和嗜酸性细胞数及细胞总数，并通过ELISA法检测肺泡灌洗液中的肿瘤坏死因子（TNF-a）、白介素一4（IL-4）、IgE、Eotaxin的水平。同时取肺组织，制备组织切片，HE染色检测组织病理学的改变。此外，还通过Westernblot法检测NF-nBp65和hcb的表达变化。【结果】和厚朴酚有效的降低了肺泡灌洗液中的总细胞数（30976．00±3865．54VS64861．67±7613．98），并同时降低了中性粒细胞、淋巴细胞、巨噬细胞和嗜酸性的细胞数，治疗前后相比较差异均具有统计学意义（P〈0．05）。ELISA检测结果也显示，和厚朴酚有效的抑制了TNF-a、IL-4、IgE、Eotaxin的表达，差异均具有统计学意义（P〈0．01）。病理学实验则显示，与模型组大面积的组织损伤和炎性细胞富集细胞相比，和厚朴酚治疗后，组织损伤得到有效缓解，炎性细胞富集减少。NF—nBp65和Ikb的表达分析显示，与模型组NF-xBp65大量表达，I-Kb表达减少相比，和厚朴酚治疗组NF—nBp65表达被显著抑制，I-kb表达显著增加，其差异均有统计学意义（P〈0．05）。【结论】和犀朴酚能够显著抑制急性哮喘诱导的炎症反应和肺组织损伤，其可能的作用机制为抑制NF-xBp65的活性。[Objective]To explore the effects of honokiol on inhibition of airway inflammation during acute asth- ma and analyze its possible mechanism. [Methods]A murine model of acute asthma was established by stimulation and induction with VOA. After a treatment of honokiol, total cells, neutrophils, Iymphocytes, macrophages and eosino- phils cells were counted. And the levels of tumor necrosis factor-alpha （TNF-a）, interleukin-4 （IL-4）, immunogloh- ulin E （IgE） and Eotaxin were detected by enzymeqinked immunosorbent assay （ELISA） in bronchoalveolar lavage fluid. Meanwhile, lung tissue was sectioned for hematoxylin ＆ eosin staining to analyze the histopathological chan- ges. Moreover, the expressionx of NF-xBp65 and Fxb were detected by Western blot. [Results]Honokiol effectively reduced the total cell number in bronchoalveolar lavage fluid （30976.00±3865.54 vs 64861.67±7613.98） while de- creased the counts of neutrophils, lymphocytes, macrophages and eosinophils count and the differences were statisti- cally significant （P 〈 0.05）. The results of ELISA showed that honokiol effectively inhibited the expressions of TNF-a, IL-4, IgE and Eotaxin. And the differences were statistically significant （ P 〈0.01）. The results of pathol- ogy showed that the area of tissue damage and inflammatory cells were effectively alleviated after a treatment of hono- kiol as compared with model group. As compared with model group, the expressions of NF-tcBp65 and I-xb decreased after a treatment of honokiol and the difference was statistically significant （ P 〈0.05）. [CondusionlHonokiol can significantly inhibit the inflammation and lung tissue damage induced by acute asthma. And its possible mechanism is through an inhibition of NF-kB activation.

关 键 词：哮喘 和厚朴酚 气道炎症 NF-KBP65 

分 类 号：R765.213[医药卫生—耳鼻咽喉科]