炙甘草汤及相应细胞培养上清对脐血BFU-E形成的影响  

Research of Influence on Prepared Licorice Decoction and Relevant Cell Culture Supernatant on BFU-E Formation

作　　者：马鹏[1] 徐秋颖[1] 刘渊[1] 

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《成都中医药大学学报》2015年第1期5-7,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81072728)

摘　　要：目的:探讨炙甘草汤及相应细胞培养上清对脐血单个核细胞BFU-E形成的影响。方法:新鲜脐血经淋巴细胞分离液分离出单个核细胞,实验采用炙甘草汤与右归饮两个中药方剂,单个核细胞设空白对照组、炙甘草汤组、右归饮组、混合组及相应细胞培养上清组,每组分别加入中药液或细胞培养上清,甲基纤维素半固体培养基培养10 d后观察集落形成情况。结果:细胞培养上清组均能刺激细胞集落形成,其中尤以炙甘草汤组最为明显,其次是混合组与右归饮组。炙甘草汤培养上清组与混合培养上清组两组与空白培养上清组相比,刺激效果明显。结论:炙甘草汤细胞培养上清能明显刺激脐血单个核细胞集落形成,对脐血细胞扩增有明显效果。Objective： To invest the effector of Prepared Licorice Decoction and relevant cell culture supernatant on BFU-E formation. Methods： Mononuclear cells were isolated from the cord blood by ficoll. Cells were divided into control group,prepared licorice decoction group,youguiyin group,mixed group and cell culture supernatant related group. The colonies were calculated 10 days after cells cultured in methyl cellulose medium. Results： All cell culture supernatant groups could stimulate colony formation. The prepared licorice decoction group do the best on stimulate colony format,and then is the mixed group and youguiyin group. Conclusion：Prepared licorice decoction can stimulate mononuclear cells form colony. The stimulate effector help the proliferation of cord blood.

关 键 词：炙甘草汤 脐静脉内皮细胞 BFU-E 

分 类 号：R289.5[医药卫生—方剂学] R322.2[医药卫生—中药学]