贵州苗药蓝布正的指纹图谱及其主要成分鞣花酸含量测定  

Fingerprint of Guizhou Miao medicine Geum japonicum and determination of its main component ellagic acid

作　　者：何迅[1] 王正[1] 王爱民 李勇军[1] 关焕玉[1] 刘俊宏[1] 廖尚高[1] 

机构地区：[1]贵阳医学院药学院,民族药与中药开发应用教育部工程研究中心,贵阳550004 [2]贵州省药物制剂重点实验室,贵阳550004

出　　处：《中国新药杂志》2015年第5期584-587,591,共5页Chinese Journal of New Drugs

基　　金：国家科技支撑计划(2013BAI11B01);贵州省中药现代化项目(黔科合中药字[2010]5026号);贵州省中药现代化科技产业研究开发专项(黔科合重G字[2013]4001号)

摘　　要：目的:建立贵州蓝布正的超高效液相色谱指纹图谱及主要成分鞣花酸的含量测定方法。方法:采用Agilent Technologies 1290 Infinity UHPLC系统测定,色谱柱:Phenomenex Synergi(2.0 mm×100 mm,2.5μm);流动相:甲醇-0.1%甲酸水溶液,梯度洗脱;流速:0.3 m L·min-1;柱温:40℃。结果:建立了贵州蓝布正的超高效液相色谱指纹图谱,标定了17个共有峰,并在相同的色谱条件下测定了24批不同产地的蓝布正药材中鞣花酸的含量,在其检测的范围内其线性关系良好,平均回收率为100.6%,RSD值为1.3%。结论:所建立的方法方便、快速、准确、重复性好,可以控制该药材的质量。Objective： To establish the UHPLC fingerprint for Guizhou Miao medicine Geum japonicum and a method for determining its main component ellagic acid. Methods： The chromatographic separation was performed on the Phenomenex Synergi column（ 2. 0 mm × 100 mm,2. 5 μm） with gradient elution of methanol and0. 1% aqueous formic acid at a flow rate of 0. 3 m L·min- 1. The column temperature was set at 40 ℃. Results：The UHPLC fingerprint with 17 common peaks was established for Geum japonicum. The method for determining ellagic acid in the same chromatographic condition was set up,which showed good linearity in the test ranges. The average recovery was 100. 6% and the RSD was 1. 3%. Conclusion： The methods developed are convenient,rapid,accurate and reproducible,which is applicable to the quality control of Guizhou Miao medicine Geum japonicum.

关 键 词：蓝布正 超高效液相色谱法 鞣花酸 含量测定 指纹图谱 

分 类 号：R927.2[医药卫生—药学]