近红外漫反射光谱结合主成分回归法快速测定黑果小檗皮中盐酸小檗碱含量  

Near Infrared Diffuse Reflection Optical Spectra Combined with PCR Rapid Determination Content of Berberine Hydrochloride in Berberis Heteropoda Cortex

作　　者：李莉[1] 王庆振[1] 孙颖平[1] 

机构地区：[1]新疆医科大学,乌鲁木齐830054

出　　处：《中国现代应用药学》2015年第3期309-314,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金(81260485)

摘　　要：目的应用近红外漫反射光谱结合主成分回归法(PCR)对黑果小檗皮中盐酸小檗碱进行快速定量分析。方法通过化学测定值与光谱间建立PCR数学模型对黑果小檗皮中盐酸小檗碱进行快速定量分析。结果黑果小檗皮中盐酸小檗碱的内部交叉验证均方差(RMSECV)为1.016 9,内部交叉验证决定系数为0.979 5,预测平均回收率为108.51%,统计学检验表明预测值与标准方法测定值之间的差异无统计学意义。结论表明利用近红外漫反射光纤光谱结合PCR对黑果小檗进行定量分析可行。OBJECTIVE To analysis berberinehydrochloride in Berberisheteropoda cortex by frared diffuse reflection optical spectra combined with PCR.METHODS Between the measured value and chemical spectroscopy,a method of NIR diffuse reflectance optic-fiber spectroscopy combined with PCR was established to analysis berberinehydrochloride in Berberisheteropoda cortex.RESULTS The root-mean-square error of cross-validation（RMSECV） of the berberinehydrochloride in Berberisheteropoda cortex was 1.016 9,the correlation coefficients was 0.979 5,the average recycle rate was 108.5%.Statistical test was used to analysis the dates and it showed that the difference between NIR prediction values and the values determinated by standard methodshad no statistical significance.CONCLUSION It indicates that NIR diffuse reflectance optic-fiber spectroscopy combined with PCR method can be used in rapid determination of the contents of Berberisheteropoda Schrenk.

关 键 词：黑果小檗 近红外光谱分析技术 主成分回归法 

分 类 号：R917.101[医药卫生—药物分析学]