利用SRAP、ISSR、SSR标记绘制黄麻基因源分子指纹图谱  

Construction of Molecular Fingerprinting Map in Gene Pool of Jute with SRAP, ISSR, and SSR Markers

作　　者：巫桂芬 徐鲜均 徐建堂[1] 陶爱芬[1] 张立武[1] 魏丽真 潘漠 方平平[1] 林荔辉[1] 祁建民[1] 

机构地区：[1]福建农林大学/作物遗传育种与综合利用教育部重点实验室/福建省作物设计育种重点实验室/福建省南方特色经济作物遗传育种与多用途综合利用国际合作基地,福建福州350002 [2]广西农业科学院园艺研究所,广西南宁530007 [3]武夷山生物资源研究所,福建武夷山3543004 [4]福州市第三中学,福建福州350002 [5]福州天地同人信息科技有限公司,福建福州350001

出　　处：《作物学报》2015年第3期367-377,共11页Acta Agronomica Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-19-E06);引进国际先进农业科学技术计划(948计划)项目(2013-Z7);国家科技支撑计划项目(2013BAD01B03-13)资助

摘　　要：以国内外引进保存的231份黄麻种质资源为材料,分别采用SRAP、ISSR、SSR分子标记和编程DNA指纹图谱分析软件,绘制黄麻遗传资源基因组DNA指纹图谱。结果表明,通过筛选出的35对SRAP引物对231份黄麻品种进行标记分析,获得96份黄麻品种DNA指纹图谱;11个ISSR多态性引物对96份材料进行DNA标记分析,获得45份黄麻品种DNA指纹图谱;49对SSR多态性引物对48份黄麻品种进行DNA标记分析,获得13份黄麻品种DNA指纹图谱,累计完成了154份黄麻品种基因组DNA分子指纹图谱绘制。每一个被识别的品种都具有其独特的分子"身份证"。其他77份地方品种因与部分品种遗传相似性过高,未能被识别,表明黄麻地方品种存在较为严重的同种异名现象。An experiment was conducted using 231 jute germplasm resources from abroad and at home to construct DNA finger-prints of jute varieties with SRAP, ISSR, SSR marker, and the DNA fingerprint analysis software. The results showed that 96 DNA fingerprints from 231 jute germplasm resources with 35 pairs of selected SRAP primers, 45 DNA fingerprints from 96 jute varieties with 11 selected polymorphic ISSR primers, and 13 DNA fingerprints from 48 jute varieties with 49 selected polymor-phic SSR primers were constructed. This study completed a total of 154 genomic DNA molecular fingerprint maps of jute varie-ties. Every identified jute variety had its unique “ID”. Other 77 local varieties had not been able to be identified due to their high genetic similarity with some varieties. It showed that jute local variety has a serious phenomenon of synonym.

关 键 词：黄麻 遗传资源 分子标记 DNA指纹图谱 

分 类 号：S563.4[农业科学—作物学]