安徽池州地区ABO新生儿溶血病检测结果分析  

Analysis of test results of ABO hemolytic disease of newborn in Chizhou area

作　　者：王从刚[1] 陈靖[1] 包建国[1] 朱书奇[1] 荣发芝 桂秀芳[1] 毛雨生[1] 

机构地区：[1]池州市人民医院输血科,安徽池州247000

出　　处：《国际检验医学杂志》2015年第5期615-616,共2页International Journal of Laboratory Medicine

摘　　要：目的了解池州地区ABO新生儿溶血病(ABO-HDN)的实验室检测现状,分析血清学三项试验检测结果,为临床诊断提供依据。方法采用微柱凝胶法对患儿进行ABO血型鉴定和血清学三项试验(直接抗人球蛋白试验、游离抗体试验、抗体释放试验)。结果患儿A、B、O、AB血型比例分别为29.13%、31.09%、37.82%、1.96%;患儿ABO-HDN阳性率为22.41%(80/357),A、B血型患儿ABO-HDN阳性率分别为38.46%(40/104)、36.04%(40/111);ABO-HDN的发生率与新生儿A或B型的型别比较差异无统计学意义(P>0.05);直接抗人球蛋白试验、游离抗体试验、抗体释放试验阳性率分别为1.96%(7/357)、4.76%(17/357)、22.41%(80/357)。结论血清学三项试验是诊断ABO-HDN的主要依据,以抗体释放试验结果阳性率最高。临床考虑新生儿溶血的患儿应及早做ABO-HDN筛查,明确诊断及早处理。Objective To understand the laboratory testing current situation of ABO hemolytic disease of the newborn（ABOHDN）in Chizhou area,and to analyze the test results of serological three indexes tests in order to provide the basis for clinical diagnosis.Methods The ABO blood group identification and serological three indexes tests（direct antiglobulin test,free antibody test,antibody release test）were performed by using microcolumn gel method.Results A,B,O and AB blood groups were 29.13%,31.09%,37.82% and 1.96%;the total positive rate of ABO-HDN was 22.41%（80/357）,the positive rates of ABO-HDN in A and B blood groups were 38.46%（40/104）and 36.04%（40/111）respectively;the occurrence rate of ABO-HDN had no statistical difference between blood group A and B（P〉0.05）;the positive rates of the direct antiglobulin test,free antibody test and antibody release test were 1.96%（7/357）,4.76%（17/357）and 22.41%（80/357）respectively.Conclusion The serological three indexes tests are the main basis for the diagnosis of ABO-HDN,the antibody release test shows the highest positive rate.If clinically considering HDN,the newborns should conduct the ABO-HDN screening as early as possible for clarifying the diagnosis and performing the early treatment.

关 键 词：ABO新生儿溶血病 直接抗人球蛋白试验 游离抗体试验 抗体释放试验 

分 类 号：R722.1[医药卫生—儿科] R446.6[医药卫生—临床医学]