复方肾炎片联合强的松对阿霉素肾病幼鼠治疗作用  

Effects of compound Shenyanpian and Prednisoneon on juvenile rats with Adriamycin-induced nephropathy

作　　者：王英娟[1,2] 潘凯丽[1] 强欢[3] 赵晓莉[1] 

机构地区：[1]第四军医大学西京医院儿科,陕西西安710032 [2]榆林市第一医院儿科,陕西榆林719000 [3]西安市儿童医院,陕西西安710003

出　　处：《中国妇幼健康研究》2015年第1期19-21,共3页Chinese Journal of Woman and Child Health Research

摘　　要：目的研究复方肾炎片联合强的松对阿霉素肾病幼鼠的治疗作用。方法 32只4周龄雄性大鼠建立阿霉素肾病模型后,随机分为模型对照组(B组)、复方肾炎片组(C组)、强的松组(D组)和联合用药组(E组),每组8只,另取8只做为正常对照组(A组)。于造模前1天、造模后2周末、4周末、8周末、12周末共5个时间点检测大鼠24h尿蛋白,12周末检测各组大鼠血白蛋白、血脂及肾功并行肾脏病理检查。结果①A组大鼠5个时间点尿蛋白水平无显著变化(F=5.03,P>0.05);B组、C组、D组、E组大鼠2周末、4周末、8周末及12周末尿蛋白较各自0周末均有显著变化(F值分别为23.59、18.17、14.53、19.61,均P<0.05)。B、C、D、E组8周末及12周末尿蛋白水平均有显著性差异(F_(周末)=15.66,F_(12周末)=25.43;均P<0.05),其中B组平均尿蛋白水平最高,8周末为(170.80±38.56)mg/24h,12周末为(141.29±36.55)rmg/24h;E组最低,8周末为(37.59±15.36)mg/24h,12周末为(12.40±4.10)mg/24h。②12周末5组间的白蛋白及总胆固醇、甘油三酯较比较,差异有统计学意义(F白蛋白=27.13、F总胆固醇=19.80、F_(甘油三酯)=19.05,均P<0.05),但其中E组白蛋白、总胆固醇、甘油三酯较A组无统计学差异(均P>0.05),5组间尿素氮及肌酐水平无统计学差异(F_(尿素氮)=10.75、F_(肌酐)=9.43、均P>0.05)。③E组相较B、C、D组有较轻病理损害,肾间质无增生,肾小管未见蛋白管型。结论复方肾炎片联合强的松治疗阿霉素肾病大鼠可显著降低尿蛋白及血脂水平,维持血清白蛋白水平,起到有效的治疗作用。Objective To explore the effect of compound Shenyanpian and Prednisoneon on juvenile rats with Adriamycin-induced nephropathy. Methods After establishing Adriamycin models, 32 4-week-old male rats were randomly divided into model control group （group B）, compound Shenyanpian group （group C） , Prednisone group （group D） and combination therapy group （group E） with 8 in each group. Another 8 were recruited in normal control group （group A）. One day before modeling and at the end of 2,4, 8 and 12 weeks after modeling 24h urine protein was detected. At the end of 12 weeks serum albumin, lipids and renal function were detected, and renal pathology examination was done. Results In group A the urine protein levels at five time points showed no significant change （ F = 5.03, P 〉 0.05 ）. Compared with the urine protein levels at 0 week, those at the end of 2,4,8 and 12 weeks were significantly different in group B, C, D and E （F value was 23.59, 18.17, 14.53 and 19.61, respectively, all P 〈0.05 ）. There were significant differences in urine protein levels at the end of 8 weeks and 12 weeks in group B, C, D and E （ F8 week = 15.66, F12 week = 25.43, both P 〈 0.05 ）. In group B the mean urine protein level was highest, 170.80 ± 38.56mg/24h at the end of 8 weeks and 141.29 ± 36.55mg/24h at 12 weeks, respectively. However, that was lowest in group E, 37.59 _＋ 15.36mg/24h at the end of 8 weeks and 12.40± 4.10mg/24h at 12 weeks, respectively. At the end of 12 weeks there were significant difference among 5 groups in albumin, total cholesterol and triglycerides （ FAlb = 27.13 ,FCho = 19.80 ,FTG = 19.05, all P 〈 0.05 ）, but group E was not significantly different from group A （all P 〉 0.05 ）. There was no significant difference among 5 groups in urea nitrogen and creatinine （ F urea nitrogen = 10. 75, Fcreatinine= 9.43, both P 〉 0.05 ）. Compared with group B, C and D, group E had less severe pathological damage. Renal interstitium showed no hyperplasia and no tubular pr

关 键 词：阿霉素 肾病综合征 复方肾炎片 幼鼠 

分 类 号：R725[医药卫生—儿科]